Yak FGF12 promotes the proliferation of fibroblasts and longissimus lumborum development

IF 3.5 3区 生物学 Q3 CELL BIOLOGY
Nanchi Zhang , Li Wang , Yong Wei , Tianwu An , Bo Ga , Lesheng Wang , Ta E
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Abstract

For a considerable period, fibroblast growth factor 12 (FGF12) was regarded as an intracellularly secreted protein, and with a paucity of research conducted on the effects of FGF12 on cell proliferation. Nevertheless, recent studies have demonstrated that FGF12 can be secreted extracellularly and exhibited bioactive properties. Given the highly conserved motifs characteristic of the FGF family that are present in FGF12, we postulated that it has the potential to stimulate cell proliferation. In this study, we validated the recombinant FGF12 protein's ability to enhance fibroblast proliferation and confer anti-apoptotic properties. The subsequent analyses were conducted using differential gene expression, KEGG pathway, RT-qPCR and Western blotting, which revealed that FGF12 stimulates fibroblast proliferation via the BMP7/Smad signaling pathway. Furthermore, in vivo experimentation with recombinant FGF12 protein revealed its potential to improve growth efficiency, augment blood glucose control and facilitate the development of longissimus lumborum in mice. Collectively, these findings afford novel insights into FGF12's unconventional secretion, its role in regulating cell proliferation, and its promise in promoting muscular development.

Abstract Image

牦牛FGF12促进成纤维细胞增殖和腰最长肌发育。
在相当长的一段时间里,成纤维细胞生长因子12 (fibroblast growth factor 12, FGF12)被认为是一种细胞内分泌的蛋白,关于FGF12对细胞增殖作用的研究较少。然而,最近的研究表明,FGF12可以在细胞外分泌,并表现出生物活性。鉴于FGF家族高度保守的基序特征存在于FGF12中,我们假设它具有刺激细胞增殖的潜力。在这项研究中,我们验证了重组FGF12蛋白增强成纤维细胞增殖和赋予抗凋亡特性的能力。随后通过差异基因表达、KEGG通路、RT-qPCR和Western blotting分析发现,FGF12通过BMP7/Smad信号通路刺激成纤维细胞增殖。此外,重组FGF12蛋白的体内实验显示其具有提高小鼠生长效率、增强血糖控制和促进腰最长肌发育的潜力。总的来说,这些发现为FGF12的非常规分泌、其在调节细胞增殖中的作用以及促进肌肉发育的前景提供了新的见解。
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来源期刊
Experimental cell research
Experimental cell research 医学-细胞生物学
CiteScore
7.20
自引率
0.00%
发文量
295
审稿时长
30 days
期刊介绍: Our scope includes but is not limited to areas such as: Chromosome biology; Chromatin and epigenetics; DNA repair; Gene regulation; Nuclear import-export; RNA processing; Non-coding RNAs; Organelle biology; The cytoskeleton; Intracellular trafficking; Cell-cell and cell-matrix interactions; Cell motility and migration; Cell proliferation; Cellular differentiation; Signal transduction; Programmed cell death.
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