Upregulation of Linc00960 promotes colorectal cancer cell proliferation by regulating hsa-miR-107/CDK6 axis

IF 3.5 3区生物学 Q3 CELL BIOLOGY

Experimental cell research Pub Date : 2025-08-01 DOI:10.1016/j.yexcr.2025.114697

Chunlin Ke , Peirong Wang , Biao Chen , Xianhui Cheng , Xinyi Zhang , Rongmu Xia , Feng Dong , Jiancheng Li

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引用次数: 0

Abstract

Colorectal cancer (CRC), the most common gastrointestinal malignancy, has incompletely understood underlying developmental mechanisms. LincRNA, a subclass of long non-coding RNAs (LncRNAs), is implicated in the pathogenesis of multiple cancers, including CRC. In this study, qPCR analysis revealed that Linc00960 expression was significantly upregulated in CRC tissues compared to adjacent non-tumor tissues and correlated with poor patient prognosis. Functional assays (including cell proliferation, colony formation, flow cytometry, Transwell assay and xenograft experiments) demonstrated that high Linc00960 expression enhanced CRC cell proliferation, whereas Linc00960 knockdown markedly suppressed cell proliferation, migration and invasion. To investigate the mechanism, dual-luciferase reporter assays were performed; there revealed that Linc00960 directly bound to hsa-miR-107 (miR-107) and negatively regulated its expression, thereby upregulating CDK6 expression and promoting CRC cell proliferation. In conclusion, our findings indicated that the Linc00960/miR-107/CDK6 axis served as a crucial signaling pathway in CRC progression, providing novel potential targets and biomarkers for CRC diagnosis and therapy.

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上调Linc00960通过调控hsa-miR-107/CDK6轴促进结直肠癌细胞增殖。

结直肠癌（CRC）是最常见的胃肠道恶性肿瘤，其潜在的发展机制尚未完全了解。lncrna是长链非编码rna （lncrna）的一个亚类，与包括CRC在内的多种癌症的发病机制有关。本研究通过qPCR分析发现，与癌旁非肿瘤组织相比，CRC组织中Linc00960的表达显著上调，且与患者预后不良相关。功能分析（包括细胞增殖、集落形成、流式细胞术、Transwell实验和异种移植实验）表明，Linc00960的高表达增强了CRC细胞的增殖，而Linc00960的低表达明显抑制了细胞的增殖、迁移和侵袭。为了研究其机制，进行了双荧光素酶报告基因检测；研究发现，Linc00960直接与hsa-miR-107 （miR-107）结合，负向调控其表达，从而上调CDK6表达，促进结直肠癌细胞增殖。总之，我们的研究结果表明，Linc00960/miR-107/CDK6轴是CRC进展的关键信号通路，为CRC的诊断和治疗提供了新的潜在靶点和生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Experimental cell research 医学-细胞生物学

CiteScore

7.20

自引率

0.00%

发文量

295

审稿时长

30 days

期刊介绍： Our scope includes but is not limited to areas such as: Chromosome biology; Chromatin and epigenetics; DNA repair; Gene regulation; Nuclear import-export; RNA processing; Non-coding RNAs; Organelle biology; The cytoskeleton; Intracellular trafficking; Cell-cell and cell-matrix interactions; Cell motility and migration; Cell proliferation; Cellular differentiation; Signal transduction; Programmed cell death.