[Prognostic significance of AIF expression in diffuse large B-cell lymphoma].

IF 0.3 4区医学 Q3 MEDICINE, GENERAL & INTERNAL

Terapevticheskii Arkhiv Pub Date : 2025-07-31 DOI:10.26442/00403660.2025.07.203269

V A Rosin, E V Vaneeva, D A Diakonov, N V Glubokovskikh, S V Samarina

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引用次数: 0

Abstract

Background: Dysregulation of apoptosis in diffuse B-large cell lymphoma (DLBCL) contributes to increased tumor proliferation, as well as the formation of resistance to treatment. One of the most relevant areas of molecular research is the study of the mechanisms of programmed cell death - apoptosis. The AIF (Apoptosis Inducing Factor) protein participates in the regulation of the caspase-independent pathway of cell death and is a potential predictor of the course of DLBCL.

Aim: To evaluate the prognostic value of AIF expression in DLBCL.

Materials and methods: A retrospective study included 100 patients with newly diagnosed DLBCL. All patients received standard 1st line therapy according to the R-CHOP scheme. Using histological, immunohistochemical studies of lymph nodes and/or other organs involved in the pathological process were performed. A morphometric calculation of the data was carried out with the determination of the percentage of AIF-expressing tumor cells. The threshold level of expression of these proteins was calculated using by the ROC analysis (57%). Based on the threshold, patients were divided into groups with high (above threshold) and low (subthreshold) protein levels. The calculation of overall (OS) and progression-free (PFS) survival was performed using the Kaplan-Meier method with graphical construction of the corresponding curves, the levels of factors were compared using the log-rank test criterion. The independent influence of factors on the course of DLBCL was determined using multivariate Cox regression analysis.

Results: The subthreshold level of AIF was associated with low rates of OS and PFS of patients (34.9 and 48.8%, respectively). As a result of multivariate Cox analysis, it was found that the AIF protein is an independent prognostic marker for the course of DLBCL.

Conclusion: The subthreshold value of AIF expression is associated with low OS and PFS in patients.

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AIF表达在弥漫性大b细胞淋巴瘤中的预后意义。

背景：弥漫性b大细胞淋巴瘤（DLBCL）的细胞凋亡失调导致肿瘤增殖增加，并形成对治疗的耐药性。细胞程序性死亡-凋亡的机制是分子研究中最相关的领域之一。AIF（凋亡诱导因子）蛋白参与caspase非依赖性细胞死亡途径的调控，是DLBCL病程的潜在预测因子。目的：探讨AIF表达在大细胞淋巴瘤中的预后价值。材料和方法：回顾性研究纳入100例新诊断的DLBCL患者。所有患者均按照R-CHOP方案接受标准一线治疗。使用组织学，免疫组织化学研究淋巴结和/或其他器官参与病理过程进行。对数据进行形态计量学计算，确定表达aif的肿瘤细胞的百分比。通过ROC分析计算这些蛋白表达的阈值水平（57%）。根据阈值将患者分为高（高于阈值）和低（低于阈值）两组。采用Kaplan-Meier法计算总生存期（OS）和无进展生存期（PFS），并绘制相应曲线，各因素水平采用log-rank检验标准进行比较。采用多因素Cox回归分析确定各因素对DLBCL病程的独立影响。结果：AIF阈下水平与患者OS和PFS发生率低相关（分别为34.9%和48.8%）。通过多变量Cox分析，我们发现AIF蛋白是DLBCL病程的独立预后标志物。结论：AIF亚阈值表达与患者低OS和PFS有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Terapevticheskii Arkhiv 医学-医学：内科

CiteScore

1.40

自引率

33.30%

发文量

171

审稿时长

3-8 weeks

期刊介绍： Терапевтический архив The journal was founded by the prominent Russian therapists M.P. Konchalovsky and G.F. Lang in 1923. Then its editors-in-chief were Professors V.N. Vinogradov and A.G. Gukasyan. Since 1972, E.I. Chazov, Academician of the Russian Academy of Sciences, has been heading the editorial board of the journal. Over 90 years, there have been more than 1000 issues where the authors and editorial staff have done their best for readers to keep abreast of current advances in medical science and practice and for physicians to master the advanced principles of recognition and treatment of a wide spectrum of visceral diseases. The papers published in the journal (editorials, original articles, lectures, reviews, etc.) cover both current scientific achievements and practical experience in diagnosing, treating, and preventing visceral diseases. The authors of publications are not only Russian, but also foreign scientists and physicians. All papers are peer-reviewed by highly qualified Russian specialists. The journal is published monthly. Traditionally, each issue has predominantly certain thematic areas covering individual therapy specializations. Every year, one of the issues is devoted to related problems in practical medicine (allergology and immunology, neurology and psychiatry, obstetrics, oncology, etc.). This all draws the attention of the reading public to the journal. The journal is indexed in RSCI (Russian Science Citation Index), PubMed/Medline, Index Medicus, Scopus/EMBASE, Web of Science Core Collection (Science Citation Index Expanded), Web of Science (Russian Science Citation Index - RSCI, Current Contents Connect, BIOSIS Previews), Google Scholar, Ulrich''s Periodicals Directory. The journal is included in the list of periodicals recommended by the Higher Attestation Committee for publishing the papers containing the basic materials of doctoral and candidate dissertations. By the decision of the Presidium of the Russian Academy of Medical Sciences, the “Therapevticheskiy Arkhiv” was awarded the Botkin medal. It was admitted to the European Association of Sciences Editors (EASE). The journal was honored with the Golden Press Fund decoration at the 13th International Press Professional Exhibition.