Fibroblasts enhance the growth and survival of adult feline small intestinal organoids.

Abstract

Intestinal organoids are important cell culture models that complement live animal studies of many intestinal pathogens. Adult feline small intestinal organoids are needed for infectious disease research but are difficult to work with due to slow growth and premature senescence. We introduce a method of co-culturing adult feline small intestinal organoids with growth-inhibited human foreskin fibroblast feeder cells to enhance organoid proliferation and survival. With feeder cells, feline jejunal and ileal organoids survived at least 9 months in culture until cryopreservation. Fibroblast supplementation increased the maximum size of cat and mouse intestinal organoids as well. The increased longevity and size of these organoids are a significant improvement on current methods. These organoids also supported pre-sexual development of the medically important parasite Toxoplasma gondii, as evidenced by expression of the merozoite-specific marker GRA11b. This GRA11b positivity was higher in mature cat organoid-derived monolayers grown for 21 days prior to infection, compared with monolayers grown for 10 days. These methods have high potential to reduce the number of cats used for infectious disease research and may be applicable for intestinal cells from other animals that are difficult to culture.IMPORTANCEMany microbial pathogens are acquired orally through contaminated food or water. Being able to model these infections in cell culture has been greatly enhanced by the development of intestinal organoid technology. One of the species that hosts several infections is cats, but cat intestinal organoids have been notoriously difficult to grow. Here, we describe a co-culture method with fibroblast cells that dramatically improves the longevity of adult cat intestinal organoids. These cat organoid cells can support the pre-sexual development stages of the intestinal pathogen Toxoplasma gondii, a parasite whose sexual cycle is restricted to cats and is the reason that pregnant women are told not to change the litter box. These culture conditions will be a resource to study other cat intestinal pathogens and intestinal organoids from other animals that are difficult to culture.