Chenglin Dai, Wenqiong Du, Na Kang, Haoyang Yang, Ting Tang, Yijun Jia, Can Chen, Zhaowen Zong
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引用次数: 0
Abstract
Background: A high activation level is a normal characteristic of lyophilized platelets (LPs); however, the effects of this activation level on the efficacy and safety of LPs after transfusion are debated.
Objectives: We aimed to test the efficacy and safety of pre-activated LPs (PLPs) in rabbits with traumatic bleeding and shock.
Methods: In vitro characteristics of PLPs, including activation level, aggregation, migration, and thromboelastography parameters, were evaluated. Limb soft tissue injury accompanied by seawater immersion and controlled hemorrhagic shock was induced in 50 rabbits, which were then divided into five groups: A (no resuscitation), B (resuscitation with Lactated Ringer's solution, LR), C (resuscitation with LR and fresh platelets), D (resuscitation with LR and LPs), and E (resuscitation with LR and PLPs pre-activated by thrombin). Blood loss, platelet count, blood urea nitrogen and lactic acid concentrations, and in vivo thromboelastography R value and maximum amplitude were recorded. Biotin-X-N-hydroxysuccinimide labeling and flow cytometry were used to measure the number of infused platelets left in circulation. Histology was used to assess whether aberrant thrombi were formed in the kidney, lung, or liver.
Results: PLPs exhibited an increased P-selectin level, enhanced aggregation, and shortened R values, with no obvious changes in migration ability or maximum amplitude. PLPs transfusion had a non-inferior effect on all in vivo parameters compared with fresh platelet transfusion, and the circulation time of PLPs was much shorter than that of fresh platelets. No obvious thrombi were found.
Conclusions: PLPs transfusion demonstrated non-inferior efficacy and safety compared with fresh platelet transfusion.
期刊介绍：
Platelets is an international, peer-reviewed journal covering all aspects of platelet- and megakaryocyte-related research.
Platelets provides the opportunity for contributors and readers across scientific disciplines to engage with new information about blood platelets. The journal’s Methods section aims to improve standardization between laboratories and to help researchers replicate difficult methods.
Research areas include:
Platelet function
Biochemistry
Signal transduction
Pharmacology and therapeutics
Interaction with other cells in the blood vessel wall
The contribution of platelets and platelet-derived products to health and disease
The journal publishes original articles, fast-track articles, review articles, systematic reviews, methods papers, short communications, case reports, opinion articles, commentaries, gene of the issue, and letters to the editor.
Platelets operates a single-blind peer review policy. Authors can choose to publish gold open access in this journal.
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