Live Cell Imaging for Keratinocyte Lineage Tracing.

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2025-08-06 DOI:10.1007/7651_2025_656

Brook Abegaze, Brooke Vittimberga, Nwamaka Ijeh, Giselle Vitcov, Richard Kim, Ruby Ghadially

引用次数: 0

Abstract

Live cell imaging is an established method that can be used to study keratinocyte division kinetics in vitro. To minimize the influence of the culture environment, unpassaged human keratinocytes can be utilized. To study the kinetics of single cells, keratinocytes are grown at clonal density and divisions are recorded utilizing time lapse photography. From this, lineage trees are constructed, allowing determination of cell cycle duration and division fates of individual keratinocytes. Here we describe a method for the culture of human keratinocytes for live cell imaging, methods for analysis of the photography, and how to assess proliferation, differentiation, and cell cycle duration.

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角质形成细胞谱系追踪的活细胞成像。

活细胞成像是一种成熟的方法，可用于研究角质细胞在体外的分裂动力学。为了尽量减少培养环境的影响，可以使用未传代的人角质形成细胞。为了研究单细胞的动力学，角化细胞在克隆密度下生长，并利用延时摄影记录分裂。由此，构建谱系树，从而确定单个角质形成细胞的细胞周期持续时间和分裂命运。在这里，我们描述了一种培养人角质形成细胞的方法，用于活细胞成像，分析摄影的方法，以及如何评估增殖，分化和细胞周期持续时间。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.