Pharmacokinetics Study of Herb-Drug Interaction of Berberine and Glipizide in Beagle Dogs Using UPLC-MS/MS.

IF 1.7 4区化学 Q3 CHEMISTRY, ANALYTICAL

International Journal of Analytical Chemistry Pub Date : 2025-07-28 eCollection Date: 2025-01-01 DOI:10.1155/ianc/7941435

He Qi, Wenjiong Wang, Xianghan Zhang, Bingyang Shang

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Abstract

Based on the establishment and validation of a UPLC-MS/MS method for detecting glipizide in beagle plasma, the herb-drug interaction (HDI) between berberine and glipizide was studied. After gradient elution separation of glipizide and internal standard, multiple reaction monitoring was used for detection in positive ion mode. The ion reactions used for quantitative analysis were glipizide m/z 446.0 ⟶ 321.0 and IS m/z 307.1 ⟶ 220.0. Six beagle dogs were treated with glipizide alone and berberine intervention, and the pharmacokinetic changes of glipizide were compared. The UPLC-MS/MS method has good linearity and the advantages of being green, simple, sensitive, and fast. After continuous administration of berberine to beagle dogs for 7 days, the pharmacokinetic process of glipizide changed with C _max, AUC_(0-t) and AUC_(0-∞) increasing, t_1/2 prolonging, and CL and Vd decreasing. When using combination therapy, attention should be paid to possible HDI.

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用UPLC-MS/MS研究小檗碱与格列吡嗪在Beagle犬体内的药动学

在建立并验证比格犬血浆中格列吡嗪检测方法的基础上，研究了小檗碱与格列吡嗪之间的药物相互作用（HDI）。格列吡嗪与内标品经梯度洗脱分离后，采用多反应监测进行正离子模式检测。用于定量分析的离子反应为格列吡嗪m/z 446.0川川市321.0和IS m/z 307.1川川市220.0。采用格列吡嗪单独治疗和小檗碱干预治疗6只beagle犬，比较格列吡嗪的药代动力学变化。UPLC-MS/MS方法线性度好，具有绿色环保、简便、灵敏、快速等优点。小猎犬连续给药7 d后，格列吡嗪的药代动力学过程发生变化，cmax、AUC（0-t）和AUC（0-∞）增加，t1/2延长，CL和Vd降低。当使用联合治疗时，应注意可能的HDI。

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来源期刊

International Journal of Analytical Chemistry CHEMISTRY, ANALYTICAL-

CiteScore

3.10

自引率

5.60%

发文量

117

期刊介绍： International Journal of Analytical Chemistry publishes original research articles that report new experimental results and methods, especially in relation to important analytes, difficult matrices, and topical samples. Investigations may be fundamental, or else related to specific applications; examples being biological, environmental and food testing, and analysis in chemical synthesis and materials processing. As well as original research, the International Journal of Analytical Chemistry also publishes focused review articles that examine the state of the art, identify emerging trends, and suggest future directions for developing fields.