Registration of three upland cotton germplasm lines resistant to root-knot and reniform nematodes

Abstract

Cotton (Gossypium hirsutum L.) germplasm lines BARBREN 713-32-28 (Reg. no. GP-1160, PI 706595), BARBREN 713-32-30 (Reg. no. GP-1161, PI 706596), and BARBREN 713-32-38 (Reg. no. GP-1162, PI 706597) were developed and released by the USDA-ARS in 2024. The objective of the releases was to provide breeders with agronomically elite germplasm that is resistant to both reniform nematode (Rotylenchulus reniformis Linford & Oliveira) and root-knot nematode [Meloidogyne incognita (Kolfoid & White) Chitwood]. The releases also reduce the severity of Fusarium wilt caused by several genotypes of Fusarium oxysporum f. sp. vasinfectum (Fov). The lines have simple sequence repeat (SSR) DNA markers CIR316_202 and BNL3661_185 for the Mi-1 and Mi-2 genes for resistance to root-knot nematode, respectively, and BNL 3279_105 and BNL 569_131 for the Ren₂^GB713 and Ren₃^GB713 genes for resistance to reniform nematode. The single nucleotide polymorphism (SNP) marker GI-187401 also was associated with the Ren₂^GB713 gene. In controlled environment assays, these lines suppressed numbers of reniform nematode eggs by a mean of 83.6% and root-knot nematode eggs by a mean of 97.2% compared to control plants. The released lines with both Mi-1 and Mi-2 genes had significantly greater shoot weight than other experimental lines with the Mi-1 gene alone, but not the Mi-2 gene in soils infested with both root-knot nematode and several genotypes of Fov race 1. The lines outyielded Fibermax ‘FM 966’ by 12% to 20% and had fiber quality equal to or greater than Phytogen ‘PSC 355’.