miR-127-3p regulates osteoporosis progression by targeting VAMP2 to modulate cell differentiation.

IF 3.9 3区 医学 Q2 ENDOCRINOLOGY & METABOLISM
Journal of Endocrinology Pub Date : 2025-08-21 Print Date: 2025-08-01 DOI:10.1530/JOE-25-0032
Jing Chen, Jing Lei, Ganggang Wang, Huiling Qin, Li Yang
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Abstract

Osteoporosis (OP) is a systemic osteopathy characterized by a decrease in bone density and mass. Human bone mesenchymal stem cells (hBMSCs) exhibit multidirectional differentiation potential and play a critical role in bone metabolism. Herein, we investigated the diagnostic potential of miR-127-3p in OP and elucidated its regulatory role in hBMSCs, thereby providing novel insights into the diagnosis and progression prediction of OP. The relative expression of miR-127-3p was measured via RT-qPCR analysis. ROC curve and logistic analysis were applied to identify the diagnostic value of miR-127-3p for OP. The CCK8 assay and flow cytometry were used to assess cell viability and apoptosis. A luciferase reporter assay was performed to assess the interaction between VAMP2 and miR-127-3p. The bone differentiation markers RUNX2, OCN, and OPN were assayed using RT-qPCR and western blotting. We observed that the expression of miR-127-3p was reduced in patients with OP, allowing it to effectively distinguish these patients from healthy individuals. Transfection with miR-127-3p mimic inhibited hBMSC apoptosis, increased cell viability, and increased RUNX2, OCN, and OPN levels. Furthermore, miR-127-3p regulated hBMSCs via targeting VAMP2. Overexpression of VAMP2 reversed the effects of miR-127-3p on apoptosis, cell viability, and bone differentiation. In conclusion, these findings suggest that miR-127-3p may be a potential diagnostic tool for OP. In addition, miR-127-3p promotes hBMSC viability and differentiation through downregulating VAMP2; this decreases OP progression. Our findings will inform new ideas for the diagnosis and developmental prediction of OP.

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miR-127-3p通过靶向VAMP2调控细胞分化调控骨质疏松进展。
骨质疏松症(OP)是一种以骨密度和骨量减少为特征的全身性骨病。人骨间充质干细胞(hBMSCs)具有多向分化潜能,在骨代谢中发挥着重要作用。在此,我们研究了miR-127-3p在OP中的诊断潜力,并阐明了其在hBMSCs中的调节作用,从而为OP的诊断和进展预测提供了新的见解。通过RT-qPCR分析测量了miR-127-3p的相对表达量。采用ROC曲线和logistic分析鉴定miR-127-3p对op的诊断价值。采用CCK8检测和流式细胞术评估细胞活力和凋亡。荧光素酶报告基因检测评估VAMP2和miR-127-3p之间的相互作用。采用RT-qPCR和western blotting检测骨分化标志物RUNX2、OCN和OPN。我们观察到miR-127-3p在OP患者中的表达降低,从而可以有效地将这些患者与健康个体区分开来。转染miR-127-3p模拟物抑制hBMSCs凋亡,增加细胞活力,增加RUNX2、OCN和OPN水平。此外,miR-127-3p通过靶向VAMP2调控hBMSCs。VAMP2过表达逆转了miR-127-3p对细胞凋亡、细胞活力和骨分化的影响。总之,这些发现表明miR-127-3p可能是OP的潜在诊断工具。此外,miR-127-3p通过下调VAMP2促进hBMSCs的活力和分化,从而减少OP的进展。我们的发现将为OP的诊断和发展预测提供新的思路。
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来源期刊
Journal of Endocrinology
Journal of Endocrinology 医学-内分泌学与代谢
CiteScore
7.90
自引率
2.50%
发文量
113
审稿时长
4-8 weeks
期刊介绍: Journal of Endocrinology is a leading global journal that publishes original research articles, reviews and science guidelines. Its focus is on endocrine physiology and metabolism, including hormone secretion; hormone action; biological effects. The journal publishes basic and translational studies at the organ, tissue and whole organism level.
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