Detection assays of mitochondrial permeability transition pore: Current status and future prospects

IF 2.4 4区生物学 Q4 CELL BIOLOGY

Acta histochemica Pub Date : 2025-08-04 DOI:10.1016/j.acthis.2025.152278

Dongyang Hong , Jiawei Huang , Sicheng Hu , Yawen Zheng , Yuhuan Wu , Ziyang Cao , Zijun Yan , Hongyang Zhang , Huanhuan Feng , Jinxia Wang , Lin Zou

引用次数: 0

Abstract

The mitochondrial permeability transition pore (mPTP) is a supramolecular entity in the inner mitochondrial membrane composed of various protein complexes, which is a critical component in maintaining mitochondrial function and cellular homeostasis. In this review, we provide a comprehensive summary of the current detection techniques for mPTP, including spectrophotometry, patch clamping, fluorescent probes, and flow cytometry, which have the potential to reveal the status of mPTP and its roles in degenerative diseases, inflammation, tumors and other diseases. Additionally, we discuss promising new methods to detect mPTP including enhancement in precision, high sensitivity, multi-parameter analysis, and technological integration. These advances highlight new possibilities of clinical diagnosis and treatment for mitochondria-related diseases.

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线粒体通透性过渡孔的检测方法：现状与展望

线粒体通透性过渡孔（mitochondrial permeability transition pore, mPTP）是线粒体内膜上由多种蛋白复合物组成的超分子实体，是维持线粒体功能和细胞稳态的关键成分。本文综述了目前mPTP的检测技术，包括分光光度法、膜片夹紧法、荧光探针法和流式细胞术等，这些技术有望揭示mPTP的状态及其在退行性疾病、炎症、肿瘤等疾病中的作用。此外，我们还讨论了有前途的检测mPTP的新方法，包括提高精度，高灵敏度，多参数分析和技术集成。这些进展突出了线粒体相关疾病的临床诊断和治疗的新可能性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta histochemica 生物-细胞生物学

CiteScore

4.60

自引率

4.00%

发文量

107

审稿时长

23 days

期刊介绍： Acta histochemica, a journal of structural biochemistry of cells and tissues, publishes original research articles, short communications, reviews, letters to the editor, meeting reports and abstracts of meetings. The aim of the journal is to provide a forum for the cytochemical and histochemical research community in the life sciences, including cell biology, biotechnology, neurobiology, immunobiology, pathology, pharmacology, botany, zoology and environmental and toxicological research. The journal focuses on new developments in cytochemistry and histochemistry and their applications. Manuscripts reporting on studies of living cells and tissues are particularly welcome. Understanding the complexity of cells and tissues, i.e. their biocomplexity and biodiversity, is a major goal of the journal and reports on this topic are especially encouraged. Original research articles, short communications and reviews that report on new developments in cytochemistry and histochemistry are welcomed, especially when molecular biology is combined with the use of advanced microscopical techniques including image analysis and cytometry. Letters to the editor should comment or interpret previously published articles in the journal to trigger scientific discussions. Meeting reports are considered to be very important publications in the journal because they are excellent opportunities to present state-of-the-art overviews of fields in research where the developments are fast and hard to follow. Authors of meeting reports should consult the editors before writing a report. The editorial policy of the editors and the editorial board is rapid publication. Once a manuscript is received by one of the editors, an editorial decision about acceptance, revision or rejection will be taken within a month. It is the aim of the publishers to have a manuscript published within three months after the manuscript has been accepted