Cancer stem cell enrichment in in vitro models: Techniques, insights, and applications

IF 10.1 1区医学 Q1 ONCOLOGY

Cancer letters Pub Date : 2025-08-05 DOI:10.1016/j.canlet.2025.217966

Fazeleh Ranjbar-Niavol , Maryam Barisam , Arezoo Khosravi , Navid Kashaninejad , Ali Zarrabi , Massoud Vosough

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Abstract

Due to the substantial role of cancer stem cells (CSCs) in therapeutic resistance and metastasis, the study of them is of great importance. However, their low abundance within the tumor cells makes it challenging to study their molecular pathophysiology. This limitation highlighted the critical need for reliable enrichment techniques to provide enough cells for in vitro tests. This review focuses on various methods that implemented for enrichment of cancer stem-like cells’ subpopulations from the heterogeneous population of cancer cells. In this regard, various key physicochemical parameters such as hypoxia, shear stress, high glucose concentration, autophagy, and chemotherapy-induced stress have been explored for their potential to enhance CSC enrichment. The results presented in the literature confirmed that modifying these parameters can improve the enrichment of CSCs by promoting stem-like cells phenotype. However, there is still a necessity for comprehensive studies that compare the impact of these parameters on CSC enrichment, which should be addressed in future works.

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肿瘤干细胞富集的体外模型：技术、见解和应用

由于肿瘤干细胞（cancer stem cells, CSCs）在治疗耐药和转移中的重要作用，对其进行研究具有重要意义。然而，它们在肿瘤细胞中的丰度很低，这给研究它们的分子病理生理带来了挑战。这一限制突出了对可靠富集技术的迫切需要，以便为体外试验提供足够的细胞。本文综述了从癌细胞异质群体中富集癌症干细胞样细胞亚群的各种方法。在这方面，各种关键的物理化学参数，如缺氧、剪切应力、高葡萄糖浓度、自噬和化疗诱导的应激，已被探索其增强CSC富集的潜力。文献结果证实，修改这些参数可以通过促进干细胞样细胞表型来改善CSCs的富集。但是，比较这些参数对CSC富集的影响仍有必要进行全面的研究，这应该在未来的工作中得到解决。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cancer letters 医学-肿瘤学

CiteScore

17.70

自引率

2.10%

发文量

427

审稿时长

15 days

期刊介绍： Cancer Letters is a reputable international journal that serves as a platform for significant and original contributions in cancer research. The journal welcomes both full-length articles and Mini Reviews in the wide-ranging field of basic and translational oncology. Furthermore, it frequently presents Special Issues that shed light on current and topical areas in cancer research. Cancer Letters is highly interested in various fundamental aspects that can cater to a diverse readership. These areas include the molecular genetics and cell biology of cancer, radiation biology, molecular pathology, hormones and cancer, viral oncology, metastasis, and chemoprevention. The journal actively focuses on experimental therapeutics, particularly the advancement of targeted therapies for personalized cancer medicine, such as metronomic chemotherapy. By publishing groundbreaking research and promoting advancements in cancer treatments, Cancer Letters aims to actively contribute to the fight against cancer and the improvement of patient outcomes.