Yüksel Akkaya, Begüm Nalça Erdin, Ahmet Münir Yılmaz, İbrahim Halil Kılıç, Zülal Aşçı Toraman
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引用次数: 0
Abstract
Background: Candida (Candidozyma) auris is a high priority fungal pathogen due to its antifungal resistance and its association with increased morbidity and mortality in infected patients.
Objectives: The aim of this study was to identify Candida species in clinical samples and to determine the clades and in vitro antifungal resistance of C. auris.
Design: Retrospective cohort.
Settings: Single-center tertiary hospital in Türkiye.
Material and methods: The study was conducted in the Medical Microbiology Laboratory of Ümraniye Training and Research Hospital between December 2023 and October 2024. Fungal samples were identified using bio-Mérieux VITEK MS v.3.2 (bio-Mérieux, France) and RT-PCR. Antifungal susceptibility testing of C. auris was performed by VITEK 2 Compact AST YS08 and SYO.
Main outcome measures: Identification of Candida species, in-vitro antifungal resistance of C. auris.
Sample size: 846 fungal isolates obtained from 746 patients were included.
Results: A total of 846 fungal isolates were identified, with C. albicans being the most common (n=440, 52%), followed by Nakaseomyces glabratus (n=124, 14.7%), C. parapsilosis (n=85, 10.1%), C. tropicalis (n=69, 8.2%) and C. auris (n=57, 6.7%). All C. auris isolates were susceptible to anidulafungin. Of these isolates, 47 (82%) were resistant to fluconazole, 34 (60%) to amphotericin B, four (7%) to caspofungin and three (5%) to micafungin. One isolate was resistant to amphotericin B, fluconazole, caspofungin and micafungin. A total of 31 (54%) isolates were resistant to amphotericin B and fluconazole. In accordance with the manufacturer's recommendations, 57 isolates were evaluated as Clade-1.
Conclusion: C. auris infections are becoming increasingly common. In order to better understand antifungal-resistance of this pathogen, advanced methods should be used for rapid detection of clades and mutations in the FKS gene should be revealed.
Limitations: Single center, whole genome sequence analysis were not performed.
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