Integration of transcriptome and metabolome reveals candidate metabolites responding to drought stress in sugarcane

IF 3 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Genomics Pub Date : 2025-07-31 DOI:10.1016/j.ygeno.2025.111092

Chaoqi Wang , Ta QuangKiet , Wenqing Li , Banghong Jian , Xiping Yang

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引用次数: 0

Abstract

Drought stress severely restricts sugarcane (Saccharum spp. cultivars) productivity, a globally vital sugar crop, yet the metabolic basis of its drought resistance remains elusive. Here, we integrated transcriptomic and metabolomic profiles of drought-stressed sugarcane to decipher the regulatory mechanisms. Using ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS), we identified 157 differentially expressed genes (DEGs) and 18 differentially accumulated metabolites (DAMs) specifically induced under drought stress. These DEGs/DAMs were co-enriched in 2-oxocarboxylic acid metabolism and branched-chain amino acid (valine/leucine/isoleucine) degradation pathways. Through weighted gene co-expression network analysis (WGCNA), we pinpointed three hub metabolites, tryptophan, proline, and 6-methylquinoline (6-MQ), exhibit a significant correlation with DEGs. Functional validation demonstrated that exogenous 6-MQ application significantly enhanced drought tolerance in sugarcane (17.6 % lower mortality) without growth penalty. Our study not only unravels a novel 6-MQ-mediated regulatory module but also provides valuable metabolite resources for improving drought resistance in sugarcane breeding.

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整合转录组和代谢组揭示甘蔗对干旱胁迫的候选代谢物。

干旱胁迫严重限制了甘蔗（Saccharum spp.栽培品种）的生产能力，但其抗旱性的代谢基础仍不清楚。在这里，我们整合了干旱胁迫甘蔗的转录组学和代谢组学特征来解读调控机制。利用超高效液相色谱-串联质谱技术（UHPLC-MS）鉴定了157个干旱胁迫下特异性诱导的差异表达基因（DEGs）和18个差异积累代谢物（dam）。这些DEGs/ dam在2-氧羧酸代谢和支链氨基酸（缬氨酸/亮氨酸/异亮氨酸）降解途径中共同富集。通过加权基因共表达网络分析（WGCNA），我们确定了三种中枢代谢物，色氨酸、脯氨酸和6-甲基喹啉（6-MQ），与DEGs具有显著相关性。功能验证表明，外源6-MQ显著提高了甘蔗的耐旱性（降低17.6% %的死亡率），且没有生长损失。我们的研究不仅揭示了一个新的6- mq介导的调控模块，而且为甘蔗育种提高抗旱性提供了宝贵的代谢物资源。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Genomics 生物-生物工程与应用微生物

CiteScore

9.60

自引率

2.30%

发文量

260

审稿时长

60 days

期刊介绍： Genomics is a forum for describing the development of genome-scale technologies and their application to all areas of biological investigation. As a journal that has evolved with the field that carries its name, Genomics focuses on the development and application of cutting-edge methods, addressing fundamental questions with potential interest to a wide audience. Our aim is to publish the highest quality research and to provide authors with rapid, fair and accurate review and publication of manuscripts falling within our scope.