{"title":"3D-floating Culture for Cancer Research: A Mini-review.","authors":"Akiko Fukuyama, Takanori Kitaguchi, Kazumasa Yoshida, Taichi Matsumoto, Kazuhiro Koikawa, Michitaka Nakano, Gen Maruta, Hisanori Maeoka, Shuhei Ishikura, Senji Shirasawa, Toshiyuki Tsunoda","doi":"10.21873/anticanres.17702","DOIUrl":null,"url":null,"abstract":"<p><p>Unlike conventional two-dimensional cell cultures, three-dimensional (3D) culture systems can more accurately replicate key gene expression and drug sensitivity features of the <i>in vivo</i> tumor environment. Of the various 3D culture techniques, 3D-floating (3DF) culture has the unique ability to facilitate high-throughput screening while enabling both visual and quantitative appraisal of the interactions between cancer and immune cells in the presence of exogenous drugs. In this study, we used this method to evaluate treatment against adhesive and non-adhesive cancer cells and found Pyra-Metho-Carnil to be a promising new anticancer drug for refractory cancers. Thus, in this study, we discuss how the use of 3DF cultures can advance cancer research.</p>","PeriodicalId":8072,"journal":{"name":"Anticancer research","volume":"45 8","pages":"3401-3408"},"PeriodicalIF":1.7000,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Anticancer research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.21873/anticanres.17702","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"ONCOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Unlike conventional two-dimensional cell cultures, three-dimensional (3D) culture systems can more accurately replicate key gene expression and drug sensitivity features of the in vivo tumor environment. Of the various 3D culture techniques, 3D-floating (3DF) culture has the unique ability to facilitate high-throughput screening while enabling both visual and quantitative appraisal of the interactions between cancer and immune cells in the presence of exogenous drugs. In this study, we used this method to evaluate treatment against adhesive and non-adhesive cancer cells and found Pyra-Metho-Carnil to be a promising new anticancer drug for refractory cancers. Thus, in this study, we discuss how the use of 3DF cultures can advance cancer research.
期刊介绍：
ANTICANCER RESEARCH is an independent international peer-reviewed journal devoted to the rapid publication of high quality original articles and reviews on all aspects of experimental and clinical oncology. Prompt evaluation of all submitted articles in confidence and rapid publication within 1-2 months of acceptance are guaranteed.
ANTICANCER RESEARCH was established in 1981 and is published monthly (bimonthly until the end of 2008). Each annual volume contains twelve issues and index. Each issue may be divided into three parts (A: Reviews, B: Experimental studies, and C: Clinical and Epidemiological studies).
Special issues, presenting the proceedings of meetings or groups of papers on topics of significant progress, will also be included in each volume. There is no limitation to the number of pages per issue.
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