Down-regulation of SMPDL3B Inhibits the Proliferation, Migration, and Invasion of Lung Adenocarcinoma Cells.

IF 1.7 4区医学 Q4 ONCOLOGY

Anticancer research Pub Date : 2025-08-01 DOI:10.21873/anticanres.17688

Jae Woong Koh, Seon-Joo Park

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引用次数: 0

Abstract

Background/aim: Sphingomyelin phosphodiesterase acid-like 3B (SMPDL3B) is an enzyme involved in lipid metabolism and cellular signaling. SMPDL3B expression is up-regulated in several cancers, suggesting its potential as a novel prognostic biomarker and therapeutic target for cancer treatment. However, its role in lung cancer remains unclear. In this study, we evaluated the biological role of SMPDL3B in lung cancer.

Materials and methods: SMPDL3B expression in lung adenocarcinoma (LUAD) tissues and cells was validated using quantitative real-time PCR and western blotting. To determine the role of SMPDL3B in LUAD cells, a small interfering RNA targeting SMPDL3B was used to suppress intracellular gene expression. Cell proliferation was performed using MTT and colony formation assays, and transwell assays with or without Matrigel were used to evaluate the role of SMPDL3B in LUAD cells migration and invasion.

Results: SMPDL3B was overexpressed in LUAD tissues and cells. Knockdown of SMPLD3B significantly inhibited cell proliferation by regulating cell cycle progression and also prevented the migration and invasion of LUAD cells.

Conclusion: SMPDL3B plays a role in LUAD, suggesting its potential as a therapeutic target for selective and personalized lung cancer treatment.

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下调SMPDL3B抑制肺腺癌细胞的增殖、迁移和侵袭

背景/目的：鞘磷脂二酯酶（Sphingomyelin phosphodiesterase acid-like 3B, SMPDL3B）是参与脂质代谢和细胞信号传导的酶。SMPDL3B在多种癌症中表达上调，表明其有潜力作为一种新的预后生物标志物和癌症治疗的治疗靶点。然而，它在肺癌中的作用尚不清楚。在本研究中，我们评估了SMPDL3B在肺癌中的生物学作用。材料与方法：采用实时荧光定量PCR和western blotting检测SMPDL3B在肺腺癌（LUAD）组织和细胞中的表达。为了确定SMPDL3B在LUAD细胞中的作用，我们使用一种靶向SMPDL3B的小干扰RNA来抑制细胞内基因表达。采用MTT法和集落形成法检测细胞增殖，采用加或不加Matrigel的transwell法评估SMPDL3B在LUAD细胞迁移和侵袭中的作用。结果：SMPDL3B在LUAD组织和细胞中过表达。敲低SMPLD3B可通过调节细胞周期进程显著抑制细胞增殖，并阻止LUAD细胞的迁移和侵袭。结论：SMPDL3B在LUAD中发挥作用，提示其可能成为肺癌选择性和个性化治疗的治疗靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Anticancer research 医学-肿瘤学

CiteScore

3.70

自引率

10.00%

发文量

566

审稿时长

2 months

期刊介绍： ANTICANCER RESEARCH is an independent international peer-reviewed journal devoted to the rapid publication of high quality original articles and reviews on all aspects of experimental and clinical oncology. Prompt evaluation of all submitted articles in confidence and rapid publication within 1-2 months of acceptance are guaranteed. ANTICANCER RESEARCH was established in 1981 and is published monthly (bimonthly until the end of 2008). Each annual volume contains twelve issues and index. Each issue may be divided into three parts (A: Reviews, B: Experimental studies, and C: Clinical and Epidemiological studies). Special issues, presenting the proceedings of meetings or groups of papers on topics of significant progress, will also be included in each volume. There is no limitation to the number of pages per issue.