Novel Stemness-Associated Scores: Enhancing Predictions of Hepatocellular Carcinoma Prognosis and Tumor Immune Microenvironment.

IF 4.1 4区医学 Q3 ONCOLOGY

Oncology Research Pub Date : 2025-07-18 eCollection Date: 2025-01-01 DOI:10.32604/or.2025.063993

Gaofeng Pan, Jiali Li, Weijie Sun, Jiayu He, Maoying Fu, Yufeng Gao

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引用次数: 0

Abstract

Aims: The aim of this study is to develop a prognostic model for hepatocellular carcinoma (HCC) using stemness-related genes (SRGs), while also pinpointing and validating pivotal genes associated with this process.

Methods: Utilizing the TCGA and ICGC database, a prognostic stemness-related scores (SRS) for HCC through a combination of WGCNA and machine learning. Bioinformatics analysis evaluated tumor immune infiltration characteristics and drug sensitivity in different SRS subgroups, identifying the key gene TOMM40L. qRT-PCR and IHC were employed to detect the expression level of TOMM40 L. Kaplan-Meier survival analysis assessed the prognostic value of TOMM40L in HCC. In vitro cell experiments explored the influence of TOMM40L on HCC cell progression and stemness.

Results: The prognostic model SRS for HCC was developed and validated, incorporating four SRGs: EIF2B4, CDCA8, TCOF1, and TOMM40L. Distinct variations in tumor immune infiltration profiles and drug sensitivity were noted across different SRS subgroups. Elevated TOMM40L levels are notably detected in malignant tissues in contrast to adjacent tissues, with heightened TOMM40L expression correlating with unfavorable prognostic outcomes. In addition, knockdown of TOMM40L significantly inhibited cell progression and stemness. Conclusion: The newly constructed SRS model is a potential biomarker for assessing HCC prognosis, and the key gene TOMM40L exhibits oncogenic properties.

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新的干细胞相关评分：增强肝细胞癌预后和肿瘤免疫微环境的预测。

目的：本研究的目的是利用干细胞相关基因（SRGs）建立肝细胞癌（HCC）的预后模型，同时也确定和验证与该过程相关的关键基因。方法：利用TCGA和ICGC数据库，通过WGCNA和机器学习相结合，获得HCC的预后相关性评分（SRS）。生物信息学分析评估了不同SRS亚组的肿瘤免疫浸润特征和药物敏感性，确定了关键基因TOMM40L。采用qRT-PCR和免疫组化检测TOMM40的表达水平，Kaplan-Meier生存分析评估TOMM40L在HCC中的预后价值。体外细胞实验探讨TOMM40L对HCC细胞进展和干细胞性的影响。结果：建立并验证了HCC预后模型SRS，包含4个SRGs: EIF2B4、CDCA8、TCOF1和TOMM40L。在不同的SRS亚组中，肿瘤免疫浸润谱和药物敏感性存在明显差异。与邻近组织相比，在恶性组织中显著检测到TOMM40L水平升高，TOMM40L表达升高与不良预后相关。此外，敲低TOMM40L显著抑制细胞的进展和干性。结论：新构建的SRS模型是评估HCC预后的潜在生物标志物，其关键基因TOMM40L具有致癌特性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Oncology Research 医学-肿瘤学

CiteScore

4.40

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： Oncology Research Featuring Preclinical and Clincal Cancer Therapeutics publishes research of the highest quality that contributes to an understanding of cancer in areas of molecular biology, cell biology, biochemistry, biophysics, genetics, biology, endocrinology, and immunology, as well as studies on the mechanism of action of carcinogens and therapeutic agents, reports dealing with cancer prevention and epidemiology, and clinical trials delineating effective new therapeutic regimens.