Spatial Organisation of Tumour cDC1 States Correlates with Effector and Stem-Like CD8+ T Cells Location

IF 3.7 3区 医学 Q2 IMMUNOLOGY
Cécile Piot, Mariana Pereira da Costa, Adi Biram, Carlos M. Minutti, Kok Haw Jonathan Lim, Mary Green, Ania Mikolajczak, Robert P. Jenkins, Lucy Meader, Michael D. Buck, Ana Cardoso, Neil Rogers, Erik Sahai, Caetano Reis e Sousa
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引用次数: 0

Abstract

CD8+ T cells are central to targeting and eliminating cancer cells. Their function is critically supported by type 1 conventional dendritic cells (cDC1s), which both prime antigen-specific CD8+ T cells in tumour-draining lymph nodes (tdLNs) and sustain primed CD8+ T cells within tumours. Despite their importance, the spatiotemporal organisation of cDC1s within tumours and their diverse functional roles remain poorly understood. Here, we use scRNAseq and unbiased spatial analysis to construct a detailed map of cDC1 states and distribution within immunogenic mouse tumours during CD8+ T-cell-mediated rejection. We reveal two distinct cDC1 activation states characterised by differential expression of genes linked to anti-tumour immunity, including Cxcl9 and Il12b. Strikingly, Il12b-expressing cDC1s are CCR7+ and enriched at tumour borders, where they closely associate with stem-like TCF1+ CD8+ T cells. In contrast, CCR7 Cxcl9-expressing cDC1s are preferentially found within the tumour parenchyma alongside effector CD8+ T cells. Analysis of a published dataset of human tumours similarly reveals a spatial association between CCR7+ cDC1 and stem-like TCF1+ CD8+ T cells. These findings uncover a highly spatially coordinated interaction between cDC1s and CD8+ T cells within tumours, shedding light on the intricate cellular dynamics that underpin effective anti-tumour immunity.

Abstract Image

肿瘤cDC1状态的空间组织与效应细胞和干细胞样CD8+ T细胞定位相关
CD8+ T细胞是靶向和消除癌细胞的核心。它们的功能受到1型常规树突状细胞(cDC1s)的关键支持,它们在肿瘤引流淋巴结(tdln)中启动抗原特异性CD8+ T细胞,并在肿瘤内维持启动CD8+ T细胞。尽管它们很重要,但cDC1s在肿瘤中的时空组织及其不同的功能作用仍然知之甚少。在这里,我们使用scRNAseq和无偏空间分析来构建cDC1状态和分布在CD8+ t细胞介导的免疫原性小鼠肿瘤中的详细地图。我们揭示了两种不同的cDC1激活状态,其特征是与抗肿瘤免疫相关的基因的差异表达,包括Cxcl9和Il12b。引人注目的是,表达il12b的cDC1s是CCR7+,在肿瘤边界富集,在那里它们与干细胞样TCF1+ CD8+ T细胞密切相关。相比之下,表达CCR7 - cxcl9的cDC1s优先存在于肿瘤实质和效应CD8+ T细胞中。对已发表的人类肿瘤数据集的分析类似地揭示了CCR7+ cDC1和干细胞样TCF1+ CD8+ T细胞之间的空间关联。这些发现揭示了肿瘤内cDC1s和CD8+ T细胞之间高度空间协调的相互作用,揭示了支撑有效抗肿瘤免疫的复杂细胞动力学。
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来源期刊
CiteScore
8.30
自引率
3.70%
发文量
224
审稿时长
2 months
期刊介绍: The European Journal of Immunology (EJI) is an official journal of EFIS. Established in 1971, EJI continues to serve the needs of the global immunology community covering basic, translational and clinical research, ranging from adaptive and innate immunity through to vaccines and immunotherapy, cancer, autoimmunity, allergy and more. Mechanistic insights and thought-provoking immunological findings are of interest, as are studies using the latest omics technologies. We offer fast track review for competitive situations, including recently scooped papers, format free submission, transparent and fair peer review and more as detailed in our policies.
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