{"title":"Effects of Atorvastatin and Gemfibrozil on Mice Corpus Cavernosum In Vitro","authors":"Ilknur Erkoseoglu, Mine Kadioglu Duman, Sabri Murat Kesim, Ersin Yaris, Nuri Ihsan Kalyoncu","doi":"10.1111/fcp.70042","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>Most of the drugs used in the treatment of cardiovascular diseases cause unfavorable effects on erectile functions. In this study, the effect of atorvastatin and gemfibrozil, which have different hypolipidemic mechanisms of action, on the erectile functions observed in mouse corpus cavernosum tissues is evaluated in vitro. Mouse corpus cavernosum tissues are dissected under ketamine and xylazine anesthesia. Vessels were suspended in 30 mL organ baths filled with Krebs solution and aerated with carbogen (95% O<sub>2</sub>, 5% CO<sub>2</sub>) at 37°C. An initial tension of 500 mg was applied to the suspended tissue strips. After a stabilization period of 90 min, the protocols were applied to the tissue. Atorvastatin and gemfibrozil showed no direct contractile or relaxant effect on corpus cavernosum tissues. Both drugs caused a dose-dependent relaxation in tissues precontracted with phenylephrine. Although the relaxant effect of atorvastatin is inhibited 40% by N-nitro-L-arginine methyl ester (L-NAME), these relaxations are totally inhibited by atropine. The relaxations caused by gemfibrozil are inhibited both by L-NAME and atropine. No change was observed in responses of the tissues to acetylcholine, nitroprusside, and electrical field stimulation when incubated with atorvastatin or gemfibrozil. As a conclusion, both drugs showed similar effects on corpus cavernosum tissues. Atorvastatin and gemfibrozil caused these effects via endothelial nitric oxide. When all the results are evaluated, not only did the two drugs show no unfavorable effects, but they may also have some beneficial effects on erectile functions.</p>\n </div>","PeriodicalId":12657,"journal":{"name":"Fundamental & Clinical Pharmacology","volume":"39 5","pages":""},"PeriodicalIF":2.5000,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Fundamental & Clinical Pharmacology","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/fcp.70042","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}
引用次数: 0
Abstract
Most of the drugs used in the treatment of cardiovascular diseases cause unfavorable effects on erectile functions. In this study, the effect of atorvastatin and gemfibrozil, which have different hypolipidemic mechanisms of action, on the erectile functions observed in mouse corpus cavernosum tissues is evaluated in vitro. Mouse corpus cavernosum tissues are dissected under ketamine and xylazine anesthesia. Vessels were suspended in 30 mL organ baths filled with Krebs solution and aerated with carbogen (95% O2, 5% CO2) at 37°C. An initial tension of 500 mg was applied to the suspended tissue strips. After a stabilization period of 90 min, the protocols were applied to the tissue. Atorvastatin and gemfibrozil showed no direct contractile or relaxant effect on corpus cavernosum tissues. Both drugs caused a dose-dependent relaxation in tissues precontracted with phenylephrine. Although the relaxant effect of atorvastatin is inhibited 40% by N-nitro-L-arginine methyl ester (L-NAME), these relaxations are totally inhibited by atropine. The relaxations caused by gemfibrozil are inhibited both by L-NAME and atropine. No change was observed in responses of the tissues to acetylcholine, nitroprusside, and electrical field stimulation when incubated with atorvastatin or gemfibrozil. As a conclusion, both drugs showed similar effects on corpus cavernosum tissues. Atorvastatin and gemfibrozil caused these effects via endothelial nitric oxide. When all the results are evaluated, not only did the two drugs show no unfavorable effects, but they may also have some beneficial effects on erectile functions.
期刊介绍:
Fundamental & Clinical Pharmacology publishes reports describing important and novel developments in fundamental as well as clinical research relevant to drug therapy. Original articles, short communications and reviews are published on all aspects of experimental and clinical pharmacology including:
Antimicrobial, Antiviral Agents
Autonomic Pharmacology
Cardiovascular Pharmacology
Cellular Pharmacology
Clinical Trials
Endocrinopharmacology
Gene Therapy
Inflammation, Immunopharmacology
Lipids, Atherosclerosis
Liver and G-I Tract Pharmacology
Metabolism, Pharmacokinetics
Neuropharmacology
Neuropsychopharmacology
Oncopharmacology
Pediatric Pharmacology Development
Pharmacoeconomics
Pharmacoepidemiology
Pharmacogenetics, Pharmacogenomics
Pharmacovigilance
Pulmonary Pharmacology
Receptors, Signal Transduction
Renal Pharmacology
Thrombosis and Hemostasis
Toxicopharmacology
Clinical research, including clinical studies and clinical trials, may cover disciplines such as pharmacokinetics, pharmacodynamics, pharmacovigilance, pharmacoepidemiology, pharmacogenomics and pharmacoeconomics. Basic research articles from fields such as physiology and molecular biology which contribute to an understanding of drug therapy are also welcomed.