Pathogen reduction diminishes the protective effects of platelets on endothelial barrier permeability in vitro

Blood Vessels, Thrombosis & Hemostasis Pub Date : 2025-08-01 DOI:10.1016/j.bvth.2025.100058

Alison B. Nair , Byron Miyazawa , Alpa Trivedi , Lindsay Vivona , Alexander Fields , Kimberly Herrera , Lucy Z. Kornblith , Joseph Cuschieri , Shibani Pati

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Abstract

Platelet transfusion not only attenuates bleeding and promotes hemostasis but also plays a critical role in vascular stability and endothelial barrier integrity. Under amotosalen-UVA pathogen reduction of platelets, pathogen nucleic acids undergo adduction, which prevents their replication and greatly reduces the risk of transfusion-transmitted infections. Although pathogen-reduced (PR) platelets are increasing in clinical use, the physiologic effects of pathogen reduction on platelets, particularly its impact on platelet-endothelial interactions, have yet to be described. This study compared PR platelets with nonpathogen-reduced (NPR) platelets in measures of effect on endothelial permeability in vitro. We hypothesized that PR platelets would be similar to NPR platelets. However, in endothelial cell immunohistochemistry and impedance assays, PR platelets demonstrated a significantly diminished capacity to attenuate endothelial barrier permeability at early storage time points. This small but significant difference requires further mechanistic and clinical study to understand its implications, particularly in patients with bleeding with vascular fragility.

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病原体减少减少血小板对内皮屏障通透性的保护作用

摘要血小板输注不仅能减轻出血、促进止血，而且对血管稳定和内皮屏障完整性起着至关重要的作用。在血小板阿莫tosalen- uva病原体减少过程中，病原体核酸发生内收，从而阻止其复制，大大降低了输血传播感染的风险。尽管病原体还原（PR）血小板在临床应用中越来越多，但病原体还原对血小板的生理效应，特别是其对血小板内皮相互作用的影响，尚未得到描述。本研究比较了PR血小板和非病原体减少（NPR）血小板在体外对内皮通透性的影响。我们假设PR血小板与NPR血小板相似。然而，在内皮细胞免疫组织化学和阻抗分析中，PR血小板在早期储存时间点显示出明显减弱内皮屏障通透性的能力。这种微小但显著的差异需要进一步的机制和临床研究来了解其含义，特别是在血管易碎性出血患者中。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Blood Vessels, Thrombosis & Hemostasis

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