Profiling Expression of BRAC2 Gene in Cases of Oral Squamous Cell Carcinoma and Oral Potentially Malignant Disorders-A Retrospective Study.

IF 0.4 Q4 SURGERY

Indian Journal of Otolaryngology and Head and Neck Surgery Pub Date : 2025-08-01 Epub Date: 2025-05-24 DOI:10.1007/s12070-025-05541-1

Arushi Tomar, Hrithik Kaushal Jain

{"title":"Profiling Expression of BRAC2 Gene in Cases of Oral Squamous Cell Carcinoma and Oral Potentially Malignant Disorders-A Retrospective Study.","authors":"Arushi Tomar, Hrithik Kaushal Jain","doi":"10.1007/s12070-025-05541-1","DOIUrl":null,"url":null,"abstract":"<p><p>Oral potentially malignant disorders (OPMD) are heterogeneous mucosal lesions of the oral cavity that have a higher chance of developing into malignancy, such as oral squamous cell carcinoma (OSCC), in contrast to their normal and other diseased counterparts. Information on diagnosed cases of OSCC and OPMDs at different grading stages was stored in the department's archives. The diagnosis was assessed and confirmed by two oral pathologists. They were sent for immunohistochemical examination after receiving their individual formalin-fixed, paraffin-embedded (FFPE) samples from the Department's archives. Accordingly, this study discovered that the BRCA2 gene, also known as the tumour suppression gene (TSG), was expressed in cases of OSCC and OPMDs. The results showed that when grade or severity increased, neither group's expression of this gene changed. We therefore conclude that a large number of studies with sample sizes would be necessary to confirm the findings, as well as to employ new tissue samples to confirm the expression of the BRCA2 gene and to incorporate methods like qPCR and Western blot (WB).</p>","PeriodicalId":49190,"journal":{"name":"Indian Journal of Otolaryngology and Head and Neck Surgery","volume":"77 8","pages":"2784-2789"},"PeriodicalIF":0.4000,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12297127/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Indian Journal of Otolaryngology and Head and Neck Surgery","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1007/s12070-025-05541-1","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/5/24 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"SURGERY","Score":null,"Total":0}

引用次数: 0

Abstract

Oral potentially malignant disorders (OPMD) are heterogeneous mucosal lesions of the oral cavity that have a higher chance of developing into malignancy, such as oral squamous cell carcinoma (OSCC), in contrast to their normal and other diseased counterparts. Information on diagnosed cases of OSCC and OPMDs at different grading stages was stored in the department's archives. The diagnosis was assessed and confirmed by two oral pathologists. They were sent for immunohistochemical examination after receiving their individual formalin-fixed, paraffin-embedded (FFPE) samples from the Department's archives. Accordingly, this study discovered that the BRCA2 gene, also known as the tumour suppression gene (TSG), was expressed in cases of OSCC and OPMDs. The results showed that when grade or severity increased, neither group's expression of this gene changed. We therefore conclude that a large number of studies with sample sizes would be necessary to confirm the findings, as well as to employ new tissue samples to confirm the expression of the BRCA2 gene and to incorporate methods like qPCR and Western blot (WB).

查看原文本刊更多论文

bracc2基因在口腔鳞状细胞癌和口腔潜在恶性疾病中的表达——一项回顾性研究

口腔潜在恶性疾病（OPMD）是一种异质性口腔粘膜病变，与正常和其他病变相比，它有更高的机会发展成恶性肿瘤，如口腔鳞状细胞癌（OSCC）。不同分级阶段的OSCC和opmd诊断病例信息保存在科室档案中。两名口腔病理学家对诊断进行了评估和确认。在收到来自该部门档案的个别福尔马林固定石蜡包埋（FFPE）样品后，将其送去进行免疫组织化学检查。因此，本研究发现BRCA2基因，也被称为肿瘤抑制基因（TSG），在OSCC和OPMDs病例中表达。结果表明，当分级或严重程度增加时，两组的该基因表达都没有改变。因此，我们得出结论，需要大量的样本量的研究来证实这些发现，并使用新的组织样本来确认BRCA2基因的表达，并结合qPCR和Western blot （WB）等方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Indian Journal of Otolaryngology and Head and Neck Surgery Medicine-Otorhinolaryngology

CiteScore

0.80

自引率

0.00%

发文量

226

审稿时长

6-12 weeks

期刊介绍： Indian Journal of Otolaryngology and Head & Neck Surgery was founded as Indian Journal of Otolaryngology in 1949 as a scientific Journal published by the Association of Otolaryngologists of India and was later rechristened as IJOHNS to incorporate the changes and progress. IJOHNS, undoubtedly one of the oldest Journals in India, is the official publication of the Association of Otolaryngologists of India and is about to publish it is 67th Volume in 2015. The Journal published quarterly accepts articles in general Oto-Rhino-Laryngology and various subspecialities such as Otology, Rhinology, Laryngology and Phonosurgery, Neurotology, Head and Neck Surgery etc. The Journal acts as a window to showcase and project the clinical and research work done by Otolaryngologists community in India and around the world. It is a continued source of useful clinical information with peer review by eminent Otolaryngologists of repute in their respective fields. The Journal accepts articles pertaining to clinical reports, Clinical studies, Research articles in basic and applied Otolaryngology, short Communications, Clinical records reporting unusual presentations or lesions and new surgical techniques. The journal acts as a catalyst and mirrors the Indian Otolaryngologist’s active interests and pursuits. The Journal also invites articles from senior and experienced authors on interesting topics in Otolaryngology and allied sciences from all over the world. The print version is distributed free to about 4000 members of Association of Otolaryngologists of India and the e-Journal shortly going to make its appearance on the Springer Board can be accessed by all the members. Association of Otolaryngologists of India and M/s Springer India group have come together to co-publish IJOHNS from January 2007 and this bondage is going to provide an impetus to the Journal in terms of international presence and global exposure.