High-resolution genome-wide profiling reveals intricate relationship between DNA damage and sperm physiology

IF 3 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Genomics Pub Date : 2025-07-26 DOI:10.1016/j.ygeno.2025.111091

Yufei Zhang , Huifen Cao , Fujian Yang , Chunlian Wang , Guihua Wang , Binghui Wang , Dingcai Lai , Mengling Fang , Zhengmian Zhang , Yong Diao , Dianliang Lin , Tenghui Zhan , Philipp Kapranov

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引用次数: 0

Abstract

Little is known about genome-wide profiles of different types of DNA damage in spermatozoa and how they relate to sperm quality. In this study, we for the first time constructed and analyzed high-resolution profiles of two very common DNA lesions in normal human sperm. We found non-random genomic distributions of the DNA lesions and a very complex relationship between DNA damage and sperm quality that depends on the type of DNA lesion and genomic location. We connected intricate details of genomic patterns of DNA damage with physiological properties of cells. We found an intriguing relationship between single-nucleotide hotspots of DNA damage and naturally occurring sequence variants, suggesting that certain regions of the genome are more prone to DNA damage which can lead to heritable sequence change. These results highlight complex relationship between DNA damage and cellular physiology which calls for more exploration of high-resolution genome-wide patterns of various DNA lesions.

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高分辨率全基因组分析揭示了DNA损伤与精子生理之间的复杂关系。

人们对精子中不同类型DNA损伤的全基因组图谱以及它们与精子质量的关系知之甚少。在这项研究中，我们首次构建并分析了正常人类精子中两种非常常见的DNA损伤的高分辨率剖面。我们发现DNA损伤的非随机基因组分布以及DNA损伤与精子质量之间非常复杂的关系，这取决于DNA损伤的类型和基因组位置。我们将DNA损伤的基因组模式的复杂细节与细胞的生理特性联系起来。我们发现DNA损伤的单核苷酸热点与自然发生的序列变异之间存在有趣的关系，这表明基因组的某些区域更容易发生DNA损伤，从而导致可遗传的序列变化。这些结果强调了DNA损伤与细胞生理学之间的复杂关系，这需要更多地探索各种DNA损伤的高分辨率全基因组模式。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Genomics 生物-生物工程与应用微生物

CiteScore

9.60

自引率

2.30%

发文量

260

审稿时长

60 days

期刊介绍： Genomics is a forum for describing the development of genome-scale technologies and their application to all areas of biological investigation. As a journal that has evolved with the field that carries its name, Genomics focuses on the development and application of cutting-edge methods, addressing fundamental questions with potential interest to a wide audience. Our aim is to publish the highest quality research and to provide authors with rapid, fair and accurate review and publication of manuscripts falling within our scope.