Optimization of a GMP-compliant automated one-pot synthesis of Al[18F]F-NOTA-Ubiquicidin29 − 41 for bacterial infection imaging by PET/CT

Abstract

AbstractSection Background

Bacterial infections and antimicrobial resistance constitute significant threats to global human health, resulting in millions of fatalities annually. The development of innovative diagnostic agents is essential to facilitate precision medicine approaches in the battle against infectious diseases. Infection imaging using radiolabeled antimicrobial peptides (AMP) has emerged as a promising approach to detect bacterial infections. UBI(29–41), an AMP fragment, exhibits binding to bacterial cell membranes. Conjugated to chelators, UBI(29–41) has been labeled with radiometals such as ^99mTc or ⁶⁸Ga, and proven its ability to differentiate between sterile inflammation and infection with S. aureus by imaging. Due to its physical properties, ¹⁸F is more favorable for PET/CT imaging. As peptide labeling with ¹⁸F is challenging, we here implemented the Al¹⁸F labeling approach. This study aims to develop an optimized, fully automated, GMP-compliant process for radiolabeling of NOTA-conjugated UBI(29–41) with Al¹⁸F for PET/CT imaging of infections.

AbstractSection Results

Optimized reaction conditions led to the establishment of a robust Al¹⁸Fcomplexation protocol, which was implemented on a SynChrom R&D module. The labeling reaction was carried out in an acidic medium (pH 4.0) at 105 °C for 15 min, followed by a two-step HPLC purification for 20 min. Optimization of reagent concentrations enabled an activity yield up to 10 ± 1 GBq, with a radiochemical yield of 24.2 ± 0.6% and an apparent molar activity of 45 ± 4 GBq/µmol at end of synthesis (EOS) (n = 3). The radiochemical purity was 96.6 ± 0.3% as determined by analytical HPLC, using UV absorption (220 nm). Quality control was successfully established using validated analytical procedures.

AbstractSection Conclusions

The developed GMP-compliant radiolabelling process yields reproducible results with sufficient activities for further translation and investigations of clinical PET/CT imaging using Al[¹⁸F]F-NOTA-UBI(29–41) in infectious diseases.