In vivo 3D myocardial membrane potential mapping in humans using PET/MRI.

IF 3.1 3区医学 Q1 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING

EJNMMI Research Pub Date : 2025-07-26 DOI:10.1186/s13550-025-01287-7

Felicitas J Bijari, Paul Kyu Han, Thibault Marin, Wonil Lee, Yanis Chemli, Inna Gertsenshteyn, Ismaël B G Mounime, Yanis Djebra, Didi Chi, Marc D Normandin, Chao Ma, Georges El Fakhri

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引用次数: 0

Abstract

Background: The mitochondrial membrane potential is a key biophysical parameter of mitochondrial function, which can be useful for the diagnosis and treatment monitoring of various cardiac diseases. We present a non-invasive PET/MR imaging method for 3D myocardial membrane potential mapping in humans.

Results: An in vivo PET/MR imaging study was performed in three healthy subjects (1 male and 2 females; 48 ± 29 years old) under a study protocol approved by the local Institutional Review Board (IRB). Written informed consent was obtained from all subjects before participation in the study. The [¹⁸F](4-Fluorophenyl)triphenylphosphonium ([¹⁸F]-FTPP⁺) PET tracer was administered using a bolus-plus-infusion protocol (bolus activity of 301.2 ± 7.6 MBq, infusion activity of 90.0 ± 4.9 MBq), where an infusion of 120 min was started shortly after the bolus injection (time of infusion, TOI). Dynamic cardiac PET/MR imaging was performed approximately 20 min after the TOI and continued for 100 min. The extracellular volume fraction mapping was performed via cardiac MR with a free-breathing, 3D cardiac T₁ mapping sequence before and after the contrast agent injection (gadoterate meglumine, 0.1 mmol/kg). A linear tangent space alignment (LTSA) model-based method was used to reconstruct high-frame-rate dynamic images from sparsely sampled (k,t)-space data for T₁. PET motion correction was performed using two steps of rigid image registration in a multi-resolution fashion, followed by a non-rigid image registration with B-spline transform. The tissue membrane potential was calculated using a kinetic model based on the Nernst equation with myocardial tracer concentration, tracer volume of distribution, and extracellular volume fraction measurements. Fully 3D membrane potential maps were successfully estimated from all three subjects. The estimated whole-heart membrane potentials were - 144.7 ± 3.5 mV, - 160.7 ± 5.3 mV, and - 165.8 ± 3.1 mV for each subject.

Conclusion: The proposed method allows 3D myocardial membrane potential mapping in humans in vivo.

Abstract Image

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利用PET/MRI进行人体体内三维心肌膜电位定位。

背景：线粒体膜电位是反映线粒体功能的重要生物物理参数，对各种心脏疾病的诊断和治疗监测具有重要意义。我们提出了一种非侵入性的PET/MR成像方法，用于人类的三维心肌膜电位制图。结果：对3名健康受试者进行了体内PET/MR成像研究(1男2女；48±29岁)，接受当地机构审查委员会（IRB）批准的研究方案。所有受试者在参与研究前都获得了书面知情同意。[18F]（4-氟苯基）三苯磷（[18F]-FTPP+） PET示踪剂采用丸加输注方案（丸活性为301.2±7.6 MBq，输注活性为90.0±4.9 MBq），在丸注射后不久开始输注120分钟（输注时间，TOI）。在TOI后约20分钟进行动态心脏PET/MR成像，并持续100分钟。在注射造影剂（gadoterate meglumine, 0.1 mmol/kg）前后，通过心脏MR自由呼吸，3D心脏T1成像序列进行细胞外体积分数制图。采用基于线性切线空间对齐（LTSA）模型的方法，从稀疏采样的（k,t）空间数据中重建T1的高帧率动态图像。PET运动校正采用多分辨率的刚性图像配准两步，然后采用b样条变换进行非刚性图像配准。采用基于能斯特方程的动力学模型，结合心肌示踪剂浓度、示踪剂分布体积和细胞外体积分数测量，计算组织膜电位。全三维膜电位图成功地估计了所有三个受试者。每位受试者全心膜电位分别为- 144.7±3.5 mV、- 160.7±5.3 mV和- 165.8±3.1 mV。结论：该方法可实现人体内心肌膜电位的三维定位。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

EJNMMI Research RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING&nb-

CiteScore

5.90

自引率

3.10%

发文量

审稿时长

13 weeks

期刊介绍： EJNMMI Research publishes new basic, translational and clinical research in the field of nuclear medicine and molecular imaging. Regular features include original research articles, rapid communication of preliminary data on innovative research, interesting case reports, editorials, and letters to the editor. Educational articles on basic sciences, fundamental aspects and controversy related to pre-clinical and clinical research or ethical aspects of research are also welcome. Timely reviews provide updates on current applications, issues in imaging research and translational aspects of nuclear medicine and molecular imaging technologies. The main emphasis is placed on the development of targeted imaging with radiopharmaceuticals within the broader context of molecular probes to enhance understanding and characterisation of the complex biological processes underlying disease and to develop, test and guide new treatment modalities, including radionuclide therapy.