LINC00634 is a novel prognostic biomarker for colon cancer associated with immune infiltration.

IF 2.5 3区医学 Q2 GASTROENTEROLOGY & HEPATOLOGY

BMC Gastroenterology Pub Date : 2025-07-25 DOI:10.1186/s12876-025-04049-w

Haiyan Wang, Yanyan Huang, Lina Zhou, Xiaotong Zhu, Xuan Ye, Yi Cen

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引用次数: 0

Abstract

Background: Colon cancer is a highly heterogeneous and most common malignant tumor of the gastrointestinal tract, which is prone to metastasis in advanced stages, leading to a poor prognosis for patients.

Methods: To gain a comprehensive understanding of genes associated with the prognosis of colon cancer, we identified a gene set (DPP7, TRPM5, ASB6, LINC00634 and GABRD) with potential prognostic value via genome-wide overall survival (OS) analysis. Further, LINC00634 was silenced by constructing small interfering RNAs (siRNAs) in Colo-320 cells.

Results: Multifactorial Cox regression analysis revealed that LINC00634, TRPM5, age, T and M stages were independent risk factors for the prognosis of colon cancer patients. Nomogram demonstrated that these risk factors had favorable predictive ability for 1-, 3- and 5-year OS in colon cancer patients. In addition, high expression of LINC00634 had a worse prognosis for colon cancer patients compared to TRPM5. In vitro experiments showed that silencing LINC00634 significantly inhibited cell proliferation and migration, and promoted cell apoptosis. Finally, immune infiltration analysis revealed that colon cancer patients with high expression of LINC00634 had lower infiltration levels of myeloid dendritic cells (mDCs) and CD8⁺ T cells in their tumor microenvironment (TME).

Conclusion: Our study found that LINC00634 can be used as a novel prognostic and immunological marker for colon cancer.

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LINC00634是一种新的结肠癌免疫浸润预后生物标志物。

背景：结肠癌是一种异质性高、最常见的胃肠道恶性肿瘤，晚期易发生转移，预后较差。方法：为了全面了解与结肠癌预后相关的基因，我们通过全基因组总生存期（OS）分析，鉴定出一组具有潜在预后价值的基因（DPP7、TRPM5、ASB6、LINC00634和GABRD）。此外，通过在Colo-320细胞中构建小干扰rna （sirna）使LINC00634沉默。结果：多因素Cox回归分析显示，LINC00634、TRPM5、年龄、T、M分期是影响结肠癌患者预后的独立危险因素。Nomogram显示这些危险因素对结肠癌患者的1、3、5年OS具有良好的预测能力。此外，与TRPM5相比，高表达的LINC00634在结肠癌患者中的预后更差。体外实验表明，沉默LINC00634可显著抑制细胞增殖和迁移，促进细胞凋亡。最后，免疫浸润分析显示，高表达LINC00634的结肠癌患者的肿瘤微环境（TME）中骨髓树突状细胞（mDCs）和CD8+ T细胞的浸润水平较低。结论：本研究发现LINC00634可作为一种新的结肠癌预后和免疫学标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

BMC Gastroenterology 医学-胃肠肝病学

CiteScore

4.20

自引率

0.00%

发文量

465

审稿时长

6 months

期刊介绍： BMC Gastroenterology is an open access, peer-reviewed journal that considers articles on all aspects of the prevention, diagnosis and management of gastrointestinal and hepatobiliary disorders, as well as related molecular genetics, pathophysiology, and epidemiology.