Bartonella henselae and Bartonella quintana antigens grown in liquid medium are inferior to cell culture-grown antigen for immunofluorescence IgG testing of patient sera.

IF 3.7 2区生物学 Q2 MICROBIOLOGY

Microbiology spectrum Pub Date : 2025-07-25 DOI:10.1128/spectrum.01405-25

Kristýna Dulavová, Iva Hammerbauerová, Kateřina Kybicová, Silke M Besier, Agnes Hillebrecht, Heike Podlich, Mareike Steyer, Wibke Ballhorn, Volkhard A J Kempf

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Abstract

Serodiagnosis of Bartonella infections predominantly relies on immunofluorescence assays (IFA) utilizing antigen preparations derived from bacteria co-cultivated with host cells. We compared the diagnostic performances of whole-cell Bartonella henselae and Bartonella quintana antigens generated in liquid culture with those from cell culture. IFA using liquid culture-derived B. henselae antigens yielded lower IgG antibody titers, and no consistent correlation in IgG titers was observed for B. quintana. We conclude that liquid-cultured Bartonella antigens are inferior to those used in commercially available diagnostics.IMPORTANCEIn this study, we compare the diagnostic performances of Bartonella henselae and Bartonella quintana antigens cultivated in liquid medium with those of antigens derived from cell culture for use in IFA of patient sera. Our results demonstrate that liquid-cultured Bartonella antigens for IFA are less reliable and may lead to false or missed diagnoses. This highlights the importance of using validated, high-quality antigens to ensure accurate and trustworthy results in clinical practice.

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在液体培养基中培养的亨塞拉巴尔通体和金塔纳巴尔通体抗原在患者血清免疫荧光IgG检测中不如细胞培养培养的抗原。

巴尔通体感染的血清学诊断主要依靠免疫荧光试验（IFA），利用从与宿主细胞共培养的细菌中提取的抗原制剂。我们比较了液体培养产生的全细胞亨塞拉巴尔通体和金塔纳巴尔通体抗原与细胞培养产生的巴尔通体抗原的诊断性能。使用液体培养来源的鸡selae抗原的IFA产生较低的IgG抗体滴度，并且在quintana中观察到IgG滴度没有一致的相关性。我们得出结论，液体培养的巴尔通体抗原不如商业诊断中使用的抗原。在这项研究中，我们比较了在液体培养基中培养的亨selae巴尔通体和quintana巴尔通体抗原与用于患者血清IFA的细胞培养抗原的诊断性能。我们的研究结果表明，液体培养的巴尔通体抗原对IFA不太可靠，可能导致误诊或漏诊。这突出了在临床实践中使用经过验证的高质量抗原以确保准确和值得信赖的结果的重要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microbiology spectrum Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.20

自引率

5.40%

发文量

1800

期刊介绍： Microbiology Spectrum publishes commissioned review articles on topics in microbiology representing ten content areas: Archaea; Food Microbiology; Bacterial Genetics, Cell Biology, and Physiology; Clinical Microbiology; Environmental Microbiology and Ecology; Eukaryotic Microbes; Genomics, Computational, and Synthetic Microbiology; Immunology; Pathogenesis; and Virology. Reviews are interrelated, with each review linking to other related content. A large board of Microbiology Spectrum editors aids in the development of topics for potential reviews and in the identification of an editor, or editors, who shepherd each collection.