Azobenzene-Modified Temperature-Responsive Short Elastin-like Peptides for Photo-Controlled Phase Transition.

IF 3.7 2区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

ACS Synthetic Biology Pub Date : 2025-07-24 DOI:10.1021/acssynbio.4c00889

Keitaro Suyama, Elissa Ngoc Mai, Iori Maeda, Takeru Nose

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引用次数: 0

Abstract

Elastin-like peptides (ELPs) have attracted attention as temperature-responsive biomaterials that can be used as drug carriers. In this study, temperature- and photoresponsive self-assembling peptide analogues were developed by conjugating short ELPs (total 20 amino acid residues) and azobenzene derivatives. The synthesized ELP-azobenzene conjugates exhibited reversible spectral changes upon UV or visible-light irradiation and temperature-responsive phase separation in aqueous solutions. The aggregation ability of the trans-isomers of the ELP-azobenzene conjugates was stronger than that of the cis-isomers. This phenomenon was attributed to the change in the hydrophilicity of the azobenzene moiety associated with photoisomerization from the trans- to the cis-isomer. In addition, aggregates of the ELP-azobenzene conjugate could be controlled by light irradiation. Therefore, this study provides a methodology for photo- and temperature-responsive ELP analogues with low molecular weights that can be easily synthesized by simple chemical reactions and are potential candidates for drug carriers that enable precise control of drug release.

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偶氮苯修饰的光控相变温度响应短弹性蛋白肽。

弹性蛋白样肽（Elastin-like peptides, ELPs）作为一种具有温度响应性的生物材料，可作为药物载体而受到广泛关注。在这项研究中，通过结合短elp（共20个氨基酸残基）和偶氮苯衍生物，开发了具有温度和光响应性的自组装肽类似物。合成的elp -偶氮苯缀合物在紫外或可见光照射和水溶液中温度响应相分离时表现出可逆的光谱变化。elp -偶氮苯共轭物的反式异构体的聚集能力强于顺式异构体。这一现象归因于偶氮苯部分的亲水性变化与光异构从反式到顺式。此外，elp -偶氮苯共轭物的聚集体可以通过光照射来控制。因此，本研究为光响应和温度响应的低分子量ELP类似物提供了一种方法，这种类似物可以通过简单的化学反应容易地合成，并且是能够精确控制药物释放的药物载体的潜在候选物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Synthetic Biology 生物-

CiteScore

8.00

自引率

10.60%

发文量

380

审稿时长

6-12 weeks

期刊介绍： The journal is particularly interested in studies on the design and synthesis of new genetic circuits and gene products; computational methods in the design of systems; and integrative applied approaches to understanding disease and metabolism. Topics may include, but are not limited to: Design and optimization of genetic systems Genetic circuit design and their principles for their organization into programs Computational methods to aid the design of genetic systems Experimental methods to quantify genetic parts, circuits, and metabolic fluxes Genetic parts libraries: their creation, analysis, and ontological representation Protein engineering including computational design Metabolic engineering and cellular manufacturing, including biomass conversion Natural product access, engineering, and production Creative and innovative applications of cellular programming Medical applications, tissue engineering, and the programming of therapeutic cells Minimal cell design and construction Genomics and genome replacement strategies Viral engineering Automated and robotic assembly platforms for synthetic biology DNA synthesis methodologies Metagenomics and synthetic metagenomic analysis Bioinformatics applied to gene discovery, chemoinformatics, and pathway construction Gene optimization Methods for genome-scale measurements of transcription and metabolomics Systems biology and methods to integrate multiple data sources in vitro and cell-free synthetic biology and molecular programming Nucleic acid engineering.