Comparison of the behavior of human lung epithelial cell lines cultured at the air-liquid interface and assessment of their responses after benzo(a)pyrene exposure

IF 2.7 3区医学 Q3 TOXICOLOGY

Toxicology in Vitro Pub Date : 2025-07-23 DOI:10.1016/j.tiv.2025.106122

Suen Boulé, Louise Verhaeghe, Dominique Courcot, Yann Landkocz

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引用次数: 0

Abstract

The biological effects of air pollution are still not well known, due to its complex mixture of particulate and gaseous compounds. In vitro cell culture models exposed at the air-liquid interface (ALI) represent a potential alternative to in vivo experiments to assess the effects of outdoor air pollution. This study compares two bronchial cell lines, Calu-3 and BEAS-2B, and two alveolar cell lines, hAELVi and A549, regarding their capacity to form a tight epithelial cell barrier for a 2-week culture period and metabolize xenobiotics actively. Culture at the air-liquid interface permits the Calu-3 and hAELVi cells to form and maintain a tight epithelial cell barrier with lower permeability to lucifer yellow, greater trans-epithelial electrical resistance, and the presence of Zonula Occludens 1 (ZO-1) protein at the membrane, than the BEAS-2B and A549 cells. Exposure to benzo(a)pyrene (BaP) induces the up-regulation of CYP1A1 and CYP1B1 genes, proteins, and functional activity at the air-liquid interface in all cell lines. So, these results demonstrate that the Calu-3 and the hAELVi cells are the more relevant models to assess the effects of ambient air pollution at the air-liquid interface, forming a tight epithelial cell barrier and being metabolically active.

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在气液界面培养的人肺上皮细胞系的行为比较及其暴露于苯并(a)芘后的反应评估

空气污染的生物效应仍然不为人所知，因为它是微粒和气体化合物的复杂混合物。暴露于空气-液体界面（ALI）的体外细胞培养模型代表了评估室外空气污染影响的体内实验的潜在替代方案。本研究比较了两种支气管细胞系Calu-3和BEAS-2B以及两种肺泡细胞系hAELVi和A549在2周培养期内形成紧密上皮细胞屏障和积极代谢外源药物的能力。与BEAS-2B和A549细胞相比，在气液界面培养使Calu-3和hAELVi细胞形成并维持紧密的上皮细胞屏障，其对路西法黄的渗透性较低，跨上皮电阻较大，膜上存在ZO-1蛋白。暴露于苯并(a)芘（BaP）可诱导所有细胞系中CYP1A1和CYP1B1基因、蛋白和气液界面功能活性的上调。因此，这些结果表明，Calu-3和hAELVi细胞在气液界面形成紧密的上皮细胞屏障并具有代谢活性，是评估环境空气污染影响的更合适的模型。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Toxicology in Vitro 医学-毒理学

CiteScore

6.50

自引率

3.10%

发文量

181

审稿时长

65 days

期刊介绍： Toxicology in Vitro publishes original research papers and reviews on the application and use of in vitro systems for assessing or predicting the toxic effects of chemicals and elucidating their mechanisms of action. These in vitro techniques include utilizing cell or tissue cultures, isolated cells, tissue slices, subcellular fractions, transgenic cell cultures, and cells from transgenic organisms, as well as in silico modelling. The Journal will focus on investigations that involve the development and validation of new in vitro methods, e.g. for prediction of toxic effects based on traditional and in silico modelling; on the use of methods in high-throughput toxicology and pharmacology; elucidation of mechanisms of toxic action; the application of genomics, transcriptomics and proteomics in toxicology, as well as on comparative studies that characterise the relationship between in vitro and in vivo findings. The Journal strongly encourages the submission of manuscripts that focus on the development of in vitro methods, their practical applications and regulatory use (e.g. in the areas of food components cosmetics, pharmaceuticals, pesticides, and industrial chemicals). Toxicology in Vitro discourages papers that record reporting on toxicological effects from materials, such as plant extracts or herbal medicines, that have not been chemically characterized.