Proteomic profiling of cerebrospinal fluid reveals pathophysiology changes and diversity of treatment response in pediatric Streptococcus pneumoniae meningitis.

IF 2.8 3区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

Expert Review of Proteomics Pub Date : 2025-09-01 Epub Date: 2025-08-03 DOI:10.1080/14789450.2025.2538654

Jing Wei, Binglin Jian, Liang Zhu, Linyun Guo, Jidong Du, Yuncui Yu, Xixi Zhang, Yunyan Wu, Wei Sun, Zhengguang Guo, Kui Zhu, Peng Guo, Lulu Jia, Gang Liu

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引用次数: 0

Abstract

Background: Streptococcus pneumoniae meningitis (SPM) is a critical pediatric infection with a high mortality rate. This study aimed to investigate changes in the cerebrospinal fluid (CSF) proteome in SPM patients and identify biomarkers for SPM diagnosis and treatment monitoring.

Research design and methods: Here, we retrospectively collected and evaluated CSF proteomes of 47 SPM and 116 non-CNS-infected patients (Control), including longitudinal samples from 11 SPM patients. Candidate biomarkers were validated by parallel reaction monitoring (PRM) in 146 samples (36 longitudinal/12 SPM, 110 Control) and evaluated via receiver operating characteristic (ROC) analysis.

Results: We identified 648 differentially expressed proteins (DEPs) associated with complement activation and oxidative stress. SPM patients with abnormal CSF white blood cells (SPMAN) exhibited dysregulation in coagulation and fibrinolysis, while those with normal counts (SPMN) displayed redox homeostasis alterations. The ELANE and H4C1 panel achieved a superior diagnostic accuracy (AUC = 0.967), and the combination of IGKV1-17, IGKC, and IGKV4-1 effectively tracked therapy response (AUC = 0.872).

Conclusion: This study establishes CSF proteomic signatures of pediatric SPM, providing dual-purpose biomarker panels for clinical diagnosis and treatment monitoring, with implications for targeted interventions.

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脑脊液蛋白质组学分析揭示了儿童肺炎链球菌脑膜炎的病理生理变化和治疗反应的多样性。

背景：肺炎链球菌脑膜炎（SPM）是一种严重的儿童感染，死亡率高。本研究旨在探讨SPM患者脑脊液（CSF）蛋白质组的变化，并确定SPM诊断和治疗监测的生物标志物。研究设计和方法：在这里，我们回顾性收集和评估了47例SPM和116例非cns感染患者（对照组）的脑脊液蛋白质组，包括11例SPM患者的纵向样本。146份样本（36份纵向/12份SPM， 110份对照）通过平行反应监测（PRM）验证候选生物标志物，并通过受试者工作特征（ROC）分析进行评估。结果：我们鉴定出648个与补体激活和氧化应激相关的差异表达蛋白（DEPs）。脑脊液白细胞（SPMAN）异常的SPM患者在凝血和纤维蛋白溶解中表现出异常，而计数正常（SPMN）的患者表现出氧化还原稳态改变。ELANE和H4C1联合检测获得了更高的诊断准确性（AUC = 0.967）， IGKV1-17、IGKC和IGKV4-1联合检测有效地跟踪了治疗反应（AUC = 0.872）。结论：本研究建立了小儿SPM的脑脊液蛋白质组学特征，为临床诊断和治疗监测提供了双重用途的生物标志物面板，具有针对性干预的意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Expert Review of Proteomics 生物-生化研究方法

CiteScore

7.60

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Expert Review of Proteomics (ISSN 1478-9450) seeks to collect together technologies, methods and discoveries from the field of proteomics to advance scientific understanding of the many varied roles protein expression plays in human health and disease. The journal coverage includes, but is not limited to, overviews of specific technological advances in the development of protein arrays, interaction maps, data archives and biological assays, performance of new technologies and prospects for future drug discovery. The journal adopts the unique Expert Review article format, offering a complete overview of current thinking in a key technology area, research or clinical practice, augmented by the following sections: Expert Opinion - a personal view on the most effective or promising strategies and a clear perspective of future prospects within a realistic timescale Article highlights - an executive summary cutting to the author''s most critical points.