{"title":"Generation of Porcine Blastoids from Embryonic Stem Cells.","authors":"Jinzhu Xiang, Hanning Wang, Duanqing Pei","doi":"10.1007/7651_2025_651","DOIUrl":null,"url":null,"abstract":"<p><p>Stem cell-based blastocyst-like structure models (blastoids) that mimic preimplantation blastocysts can be used to study embryogenesis and key early embryonic developmental events. Large animals may benefit from blastoid models for purposes such as improving and accelerating breeding. We developed a three-dimensional (3D) two-step differentiation strategy to generate porcine blastoids from embryonic stem cells (ESCs). The ESC-derived blastoids exhibit similar morphology, cell lineage composition, and single-cell transcriptome characteristics to porcine blastocysts. These porcine blastoids can survive and expand for more than two weeks in vitro under two different culture conditions. Here, we describe a step-by-step protocol for the generation of porcine blastoids from ESCs.</p>","PeriodicalId":18490,"journal":{"name":"Methods in molecular biology","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Methods in molecular biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1007/7651_2025_651","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 0
Abstract
Stem cell-based blastocyst-like structure models (blastoids) that mimic preimplantation blastocysts can be used to study embryogenesis and key early embryonic developmental events. Large animals may benefit from blastoid models for purposes such as improving and accelerating breeding. We developed a three-dimensional (3D) two-step differentiation strategy to generate porcine blastoids from embryonic stem cells (ESCs). The ESC-derived blastoids exhibit similar morphology, cell lineage composition, and single-cell transcriptome characteristics to porcine blastocysts. These porcine blastoids can survive and expand for more than two weeks in vitro under two different culture conditions. Here, we describe a step-by-step protocol for the generation of porcine blastoids from ESCs.
期刊介绍：
For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.
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