Exploring the role of catalytic triad Ser-His-Asp on the dynamics of the Lactobacillus helveticus cell-envelope proteinases

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Enzyme and Microbial Technology Pub Date : 2025-07-20 DOI:10.1016/j.enzmictec.2025.110723

Li Chen , Ni Lei , Guanli Du , He Chen , Guowei Shu

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引用次数: 0

Abstract

Lactic acid bacterium (LAB) hydrolyzes milk proteins into small bioactive peptides to flourish milk nutrition value. Cell-envelope proteinases (CEPs) are vitally important to bacterial growth, the texture and flavor formation, and the generation of bioactive peptides in fermented milk. Previous literature suggested PR domain of CEP was responsible for the catalytic activity. This study aims to explore the CEP molecular mechanism by delineating the catalytic triad Ser-His-Asp active sites of PR domain in Lactobacillus helveticus CNRZ32 with the aid of homology modeling, molecular docking and dynamics analysis. These results proved that catalytic triads were involved in the PR activation and the catalytic residues Ser608 and His270 appeared to be the core of catalytic process. Our study gained novel insights on the catalytic mechanism of CEP of L. helveticus CNRZ32 which would be a pioneer to facilitate the development of dairy product industry.

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探讨催化三元体Ser-His-Asp在helveticus乳杆菌胞膜蛋白酶动力学中的作用

乳酸菌（LAB）可以将牛奶蛋白水解成具有生物活性的小肽，从而提高牛奶的营养价值。细胞包膜蛋白酶（cep）对发酵乳中细菌的生长、质地和风味的形成以及生物活性肽的产生至关重要。以往文献认为CEP的PR结构域与催化活性有关。本研究旨在通过同源建模、分子对接和动力学分析，对helveticus Lactobacillus CNRZ32中PR结构域的催化三联体Ser-His-Asp活性位点进行刻画，探讨CEP的分子机制。这些结果证明，催化三联体参与了PR活化，催化残基Ser608和His270似乎是催化过程的核心。本研究对乳酸菌CNRZ32的CEP催化机理有了新的认识，为乳制品工业的发展奠定了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Enzyme and Microbial Technology 生物-生物工程与应用微生物

CiteScore

7.60

自引率

5.90%

发文量

142

审稿时长

38 days

期刊介绍： Enzyme and Microbial Technology is an international, peer-reviewed journal publishing original research and reviews, of biotechnological significance and novelty, on basic and applied aspects of the science and technology of processes involving the use of enzymes, micro-organisms, animal cells and plant cells. We especially encourage submissions on: Biocatalysis and the use of Directed Evolution in Synthetic Biology and Biotechnology Biotechnological Production of New Bioactive Molecules, Biomaterials, Biopharmaceuticals, and Biofuels New Imaging Techniques and Biosensors, especially as applicable to Healthcare and Systems Biology New Biotechnological Approaches in Genomics, Proteomics and Metabolomics Metabolic Engineering, Biomolecular Engineering and Nanobiotechnology Manuscripts which report isolation, purification, immobilization or utilization of organisms or enzymes which are already well-described in the literature are not suitable for publication in EMT, unless their primary purpose is to report significant new findings or approaches which are of broad biotechnological importance. Similarly, manuscripts which report optimization studies on well-established processes are inappropriate. EMT does not accept papers dealing with mathematical modeling unless they report significant, new experimental data.