AlliGator: Open source fluorescence lifetime imaging analysis in G

IF 2.4 4区计算机科学 Q2 COMPUTER SCIENCE, SOFTWARE ENGINEERING

SoftwareX Pub Date : 2025-07-22 DOI:10.1016/j.softx.2025.102255

Xavier Michalet

引用次数: 0

Abstract

Fluorescence Lifetime Imaging (FLI) is a technique recording the temporal decay of fluorescence emission at every pixel of an image. Analyzing the information embedded in FLI dataset requires either fitting the decay to a predefined model using nonlinear least-square fit or maximum likelihood estimation, or projecting the decay on an orthogonal basis of periodic functions as in phasor analysis. AlliGator is a Windows open source software (BSD license) implementing these approaches in a user-friendly graphical user interface (GUI) and offering numerous unique features such as Förster Resonant Energy Transfer (FRET) stoichiometry analysis, or the creation of maps of lifetime-derived quantities such as membrane potential. It leverages the unique ability of the LabVIEW graphical programming language (G) to design feature-rich GUI, and supports user-developed plugins written in python to extend its native capabilities.

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短吻鳄：开源荧光寿命成像分析

荧光寿命成像（FLI）是一种记录图像中每个像素的荧光发射时间衰减的技术。分析FLI数据集中嵌入的信息需要使用非线性最小二乘拟合或最大似然估计将衰减拟合到预定义模型，或者在相量分析中将衰减投影到周期函数的正交基础上。AlliGator是一个Windows开源软件（BSD许可），在用户友好的图形用户界面（GUI）中实现了这些方法，并提供了许多独特的功能，如Förster共振能量传递（FRET）化学计量分析，或创建生命周期衍生量的地图，如膜电位。它利用LabVIEW图形编程语言(G)的独特能力来设计功能丰富的GUI，并支持用python编写的用户开发插件来扩展其本地功能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

SoftwareX COMPUTER SCIENCE, SOFTWARE ENGINEERING-

CiteScore

5.50

自引率

2.90%

发文量

184

审稿时长

9 weeks

期刊介绍： SoftwareX aims to acknowledge the impact of software on today''s research practice, and on new scientific discoveries in almost all research domains. SoftwareX also aims to stress the importance of the software developers who are, in part, responsible for this impact. To this end, SoftwareX aims to support publication of research software in such a way that: The software is given a stamp of scientific relevance, and provided with a peer-reviewed recognition of scientific impact; The software developers are given the credits they deserve; The software is citable, allowing traditional metrics of scientific excellence to apply; The academic career paths of software developers are supported rather than hindered; The software is publicly available for inspection, validation, and re-use. Above all, SoftwareX aims to inform researchers about software applications, tools and libraries with a (proven) potential to impact the process of scientific discovery in various domains. The journal is multidisciplinary and accepts submissions from within and across subject domains such as those represented within the broad thematic areas below: Mathematical and Physical Sciences; Environmental Sciences; Medical and Biological Sciences; Humanities, Arts and Social Sciences. Originating from these broad thematic areas, the journal also welcomes submissions of software that works in cross cutting thematic areas, such as citizen science, cybersecurity, digital economy, energy, global resource stewardship, health and wellbeing, etcetera. SoftwareX specifically aims to accept submissions representing domain-independent software that may impact more than one research domain.