Single-Cell Transcriptome Analyses of Four Pain Related Genes in Osteosarcoma.

IF 2.4 Q2 MATHEMATICAL & COMPUTATIONAL BIOLOGY

Cancer Informatics Pub Date : 2025-07-19 eCollection Date: 2025-01-01 DOI:10.1177/11769351251331508

Mesalie Feleke, Haiyingjie Lin, Yun Liu, Liang Mo, Emel Rothzerg, Dezhi Song, Jinmin Zhao, Wenyu Feng, Jiake Xu

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引用次数: 0

Abstract

Objective: Osteosarcoma (OS) is a rare and complex form of cancer that mostly affects children and adolescents. Pain is a common symptom for patients in OS which causes significant unhappiness and persistent aches. To date, there is minimal knowledge on the mechanisms underlying OS induced pain and few treatment options for patients. Previous genetic studies have demonstrated that the panel of four genes, artemin (ARTN), persephin (PSPN), glial cell line-derived neurotropic factor (GDNF), and neurturin (NRTN) are associated with the regulation of pain processing in OS and analgesic responses.

Methods: In the present study, by utilising a scRNA-seq OS dataset, we aimed to measure the gene expression levels of four pain related genes, and compare them between the different cell types in human OS tissues and cell lines.

Results: Within a complex and diverse range of cell types in OS tissues, including osteoblastic OS cells, carcinoma associated fibroblasts (CAFs), B cells, myeloid cells 1, myeloid cells 2, NK/T cells, plasmocytes, ARTN and NRTN genes had the highest expression in Osteoblastic OS cells, GDNF gene had a peak expression in carcinoma associated fibroblasts, and PSPN gene in endothelial cells. In addition, all four genes showed deferential pattern of expression in 16 OS cell lines.

Conclusion: Future studies should investigate the potential to target deferentially expressed pain-related genes in specific cell types of OS for therapeutic benefit to improve the quality of life for patients living with pain caused by OS.

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骨肉瘤中4个疼痛相关基因的单细胞转录组分析。

目的：骨肉瘤（OS）是一种罕见而复杂的癌症，主要发生在儿童和青少年。疼痛是OS患者的常见症状，它会导致严重的不愉快和持续的疼痛。迄今为止，对OS引起疼痛的机制知之甚少，对患者的治疗选择也很少。先前的遗传学研究已经证明，artemin （ARTN）、persephin （PSPN）、胶质细胞系衍生的神经营养因子（GDNF）和neurturin （NRTN）这四个基因组合与OS中的疼痛加工和镇痛反应的调节有关。方法：在本研究中，我们利用scRNA-seq OS数据集，旨在测量四种疼痛相关基因的基因表达水平，并在不同细胞类型的人类OS组织和细胞系中进行比较。结果：骨肉瘤组织中细胞类型复杂多样，成骨骨肉瘤细胞、癌相关成纤维细胞（CAFs）、B细胞、髓样细胞1、髓样细胞2、NK/T细胞、浆细胞中，ARTN和NRTN基因在成骨骨肉瘤细胞中表达最高，GDNF基因在癌相关成纤维细胞中表达最高，PSPN基因在内皮细胞中表达最高。此外，这4个基因在16株OS细胞株中均表现出恭顺的表达模式。结论：未来的研究应探讨在特定细胞类型的骨肉瘤中特异性表达的疼痛相关基因的治疗效果，以改善骨肉瘤引起的疼痛患者的生活质量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cancer Informatics Medicine-Oncology

CiteScore

3.00

自引率

5.00%

发文量

审稿时长

8 weeks

期刊介绍： The field of cancer research relies on advances in many other disciplines, including omics technology, mass spectrometry, radio imaging, computer science, and biostatistics. Cancer Informatics provides open access to peer-reviewed high-quality manuscripts reporting bioinformatics analysis of molecular genetics and/or clinical data pertaining to cancer, emphasizing the use of machine learning, artificial intelligence, statistical algorithms, advanced imaging techniques, data visualization, and high-throughput technologies. As the leading journal dedicated exclusively to the report of the use of computational methods in cancer research and practice, Cancer Informatics leverages methodological improvements in systems biology, genomics, proteomics, metabolomics, and molecular biochemistry into the fields of cancer detection, treatment, classification, risk-prediction, prevention, outcome, and modeling.