Fusobacterium nucleatum and IL-32 co-predict nodal metastasis preoperatively in head and neck cancer.

IF 1.7 4区 医学 Q4 ONCOLOGY
Translational cancer research Pub Date : 2025-06-30 Epub Date: 2025-06-19 DOI:10.21037/tcr-2024-2539
Zhenwei Wang, Huiying Huang, Xiaohui Yuan, Chiyao Hsueh, Ming Zhang
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引用次数: 0

Abstract

Background: Head and neck squamous cell carcinoma (HNSCC) is a common malignancy with high rate of lymph node metastasis (LNM), which significantly impacts prognosis. This study aims to evaluate the potential utility of Fusobacterium nucleatum (F. nucleatum) and interleukin-32 (IL-32) as co-predictors for cervical LNM in HNSCC, enhancing preoperative assessment of metastatic status in patients.

Methods: Clinical information, tissue, and plasma samples were collected from a cohort of 254 HNSCC patients. IL-32 expression was assessed using immunohistochemistry (IHC), while fluorescence in situ hybridization (FISH) was used to evaluate F. nucleatum enrichment in both tumor and paracancer tissue. Univariate and multivariate analyses identified significant risk factors.

Results: IL-32 levels were higher in tumor tissue compared to paracancer tissue (P<0.001). Patients with high F. nucleatum enrichment had higher IL-32 expression (P=0.04). Through univariate and multivariate analyses, alcohol, Ki-67 (antigen Kiel-67) expression, F. nucleatum enrichment and IL-32 expression were identified as significant risk factors of LNM. The comparison of receiver operating characteristic (ROC) curves for these factors showed that the combined use of all chosen indicators resulted in the highest diagnostic accuracy.

Conclusions: The enrichment of F. nucleatum in cancer tissue is positively correlated with high expression of IL-32, suggesting that these two novel indicators, F. nucleatum and IL-32, along with Ki-67 and alcohol, may serve as preoperative predictors for LNM in HNSCC. This discovery holds great potential to facilitate the development of more precise and personalized surgical strategies for patients.

核梭杆菌和IL-32共同预测头颈部癌术前淋巴结转移。
背景:头颈部鳞状细胞癌(HNSCC)是一种常见的恶性肿瘤,淋巴结转移率高,对预后有重要影响。本研究旨在评估核梭杆菌(F. nucleatum)和白细胞介素-32 (IL-32)作为HNSCC颈部淋巴结转移的共同预测因子的潜在效用,增强患者术前转移状态的评估。方法:收集254例HNSCC患者的临床资料、组织和血浆样本。采用免疫组织化学(IHC)检测IL-32的表达,荧光原位杂交(FISH)检测核梭菌在肿瘤和癌旁组织中的富集程度。单因素和多因素分析确定了显著的危险因素。结果:肿瘤组织中IL-32水平高于癌旁组织,核核富集和IL-32表达是LNM的重要危险因素。这些因素的受试者工作特征(ROC)曲线的比较表明,所有选定指标的联合使用导致最高的诊断准确性。结论:癌组织中F. nucleatum的富集与IL-32的高表达呈正相关,提示F. nucleatum和IL-32这两个新的指标以及Ki-67和酒精可能作为HNSCC LNM的术前预测指标。这一发现具有巨大的潜力,有助于为患者制定更精确和个性化的手术策略。
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来源期刊
CiteScore
2.10
自引率
0.00%
发文量
252
期刊介绍: Translational Cancer Research (Transl Cancer Res TCR; Print ISSN: 2218-676X; Online ISSN 2219-6803; http://tcr.amegroups.com/) is an Open Access, peer-reviewed journal, indexed in Science Citation Index Expanded (SCIE). TCR publishes laboratory studies of novel therapeutic interventions as well as clinical trials which evaluate new treatment paradigms for cancer; results of novel research investigations which bridge the laboratory and clinical settings including risk assessment, cellular and molecular characterization, prevention, detection, diagnosis and treatment of human cancers with the overall goal of improving the clinical care of cancer patients. The focus of TCR is original, peer-reviewed, science-based research that successfully advances clinical medicine toward the goal of improving patients'' quality of life. The editors and an international advisory group of scientists and clinician-scientists as well as other experts will hold TCR articles to the high-quality standards. We accept Original Articles as well as Review Articles, Editorials and Brief Articles.
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