Inhibition of KIAA0101 Expression Can Regulate Cell Proliferation and Apoptosis in Colon Cancer.

Abstract

Background: Colon cancer is a prevalent malignant tumor of digestive tract occurring in the colon. In the people with cancer, colon cancer is the third leading cause of deaths in the world. Basic research on colon cancer is of great importance to patients with the disease. Methods: In this article, the authors observed the KIAA0101 expression in different types of cancers in public database (GEPIA2), found a gene network associated with KIAA0101 in STRING database, and explored the correlation of these genes with KIAA0101 at the expression level and the immune cells associated with the expression of KIAA0101. Then the authors detected the affection of KIAA0101 to the proliferation and apoptosis of cancer cells and verified the consistency of KIAA0101 expression in 31 tumor patient tissues using the database. Results: KIAA0101 is differentially expressed in all kinds of tumor cells, and it shows high expression in colorectal cancer tissues. The authors found 10 genes related to KIAA0101, including CDC20, TOP2A, CCNB2, CCNA2, and so on, all of which show positive correlation with KIAA0101 expression. In addition, the result of this study showed that KIAA0101 expression had a positive correlation with the infiltration of Th1 and Th2 cells, with correlation coefficients of 0.371 and 0.627, respectively. KIAA0101 gene shows high expression in both HCT15 and SW480 cell lines. Inhibition of KIAA0101 expression can increase caspase 3/7 activity in both HCT15 and SW480 cell lines, inhibit the proliferation ability of the two cell lines, and inhibit Bcl-2 gene expression. KIAA0101 also has tumor-suppressing effects in mice. The expression of KIAA0101 gene also shows a significant increase in the tissues collected from 31 patients with tumor. Conclusions: Inhibition of KIAA0101 expression can regulate cell proliferation and apoptosis in colorectal cancer.