Mapping of volatile organic compounds in children's saliva using headspace sorbent extraction coupled to TD-GC-MS.

IF 2.8 3区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

Expert Review of Proteomics Pub Date : 2025-08-01 Epub Date: 2025-07-24 DOI:10.1080/14789450.2025.2537254

Efstathios A Elia, Anna Zissimou, Photini Papaioakeim, Agapios Agapiou

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引用次数: 0

Abstract

Objectives: Saliva, an easily accessible biofluid, has emerged as a promising source of biomarkers for noninvasive disease diagnostics. Human saliva comprises a complex mixture of volatile organic compounds (VOCs) originating from various sources, including exhaled breath, diet, environmental factors, and the body's metabolic activity. Their presence in saliva can be influenced by several factors, including age, gender, diet, lifestyle, health status, and the oral microbiome.

Methods: Qualitative analysis of salivary VOCs as potential indicators of specific lifestyles and early environmental exposure in children was performed. Saliva samples from 40 children were analyzed using a headspace high-capacity sorptive extraction (HS-HiSorb) technique coupled with thermal desorption-gas chromatography-mass spectrometry (TD-GC-MS). The method was developed and conditions such as the choice of sorbent material and sample preparation steps were optimized.

Results: A range of VOCs in the saliva samples, including alcohols, aldehydes, ketones, nitrogen and sulfur containing compounds, organic acids, esters and hydrocarbons were identified and a selection of them were quantified. The influence of the HiSorb coating composition and the extraction parameters on the observed chromatographic areas of each analyte were also evaluated.

Conclusion: A green sample preparation method for the qualitative analysis of VOCs in the saliva of children was developed.

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顶空吸附剂萃取联用TD-GC-MS测定儿童唾液中挥发性有机化合物。

目的：唾液是一种易于获取的生物流体，已成为无创疾病诊断中有前途的生物标志物来源。人类唾液是挥发性有机化合物（VOCs）的复杂混合物，其来源多种多样，包括呼出的气体、饮食、环境因素和人体的代谢活动。它们在唾液中的存在受到几个因素的影响，包括年龄、性别、饮食、生活方式、健康状况和口腔微生物群。方法：定性分析儿童唾液挥发性有机化合物作为特定生活方式和早期环境暴露的潜在指标。采用顶空高容量吸附萃取（HS-HiSorb）技术结合热解吸-气相色谱-质谱（TD-GC-MS）对40例儿童的唾液样本进行分析。建立了该方法，并对吸附材料的选择、样品制备步骤等条件进行了优化。结果：在唾液样本中发现了一系列挥发性有机化合物，包括醇类、醛类、酮类、含氮和含硫化合物、有机酸、酯类和碳氢化合物，并对其中的一部分进行了量化。分析了HiSorb涂层组成和萃取参数对各分析物色谱面积的影响。结论：建立了一种定量分析儿童唾液中挥发性有机化合物的绿色样品制备方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Expert Review of Proteomics 生物-生化研究方法

CiteScore

7.60

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Expert Review of Proteomics (ISSN 1478-9450) seeks to collect together technologies, methods and discoveries from the field of proteomics to advance scientific understanding of the many varied roles protein expression plays in human health and disease. The journal coverage includes, but is not limited to, overviews of specific technological advances in the development of protein arrays, interaction maps, data archives and biological assays, performance of new technologies and prospects for future drug discovery. The journal adopts the unique Expert Review article format, offering a complete overview of current thinking in a key technology area, research or clinical practice, augmented by the following sections: Expert Opinion - a personal view on the most effective or promising strategies and a clear perspective of future prospects within a realistic timescale Article highlights - an executive summary cutting to the author''s most critical points.