Contemplating Bioproduction of Type-I Insect Sex Pheromones in Saccharomyces cerevisiae.

IF 3.7 2区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

ACS Synthetic Biology Pub Date : 2025-07-18 DOI:10.1021/acssynbio.5c00314

Shimin Wu, Xiao Lian, Xizhen Ge, Pingfang Tian

引用次数: 0

Abstract

Insect sex pheromones (ISPs) can act as baits to trap heterosexual insects, representing a promising biocontrol strategy. However, current industrial production of ISPs relies largely on conventional chemical synthesis, which shows drawbacks, such as low yield and environmental pollution. Fortunately, remarkable progress in metabolic pathway engineering opens the possibility of biomanufacturing ISPs in microbial cell factories, which seems to be superior to chemical synthesis. While previous reviews have summarized pathways and enzymes related to the biosynthesis of ISPs, this review zeroes in on the enzymatic structures and catalytic mechanisms. In particular, we propose a potential pathway and discuss strategies for the heterologous biosynthesis of type-I ISPs in Saccharomyces cerevisiae, a suitable host for expressing enzymes, especially those requiring post-translational modifications. Overall, this review seeks to provide valuable insights for the complete biosynthesis of type-I ISPs.

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酿酒酵母生产i型昆虫性信息素的研究。

昆虫性信息素（ISPs）可以作为诱捕异性昆虫的诱饵，是一种很有前途的生物防治策略。然而，目前的工业生产主要依赖于传统的化学合成，这显示出收率低和环境污染等缺点。幸运的是，代谢途径工程的显著进展开启了微生物细胞工厂生物制造isp的可能性，这似乎优于化学合成。虽然以前的综述综述了与ISPs生物合成相关的途径和酶，但本综述的重点是酶的结构和催化机制。特别是，我们提出了一种潜在的途径，并讨论了在酿酒酵母中异种生物合成i型ISPs的策略，酿酒酵母是表达酶的合适宿主，特别是那些需要翻译后修饰的酶。总之，本综述旨在为i型isp的完全生物合成提供有价值的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Synthetic Biology 生物-

CiteScore

8.00

自引率

10.60%

发文量

380

审稿时长

6-12 weeks

期刊介绍： The journal is particularly interested in studies on the design and synthesis of new genetic circuits and gene products; computational methods in the design of systems; and integrative applied approaches to understanding disease and metabolism. Topics may include, but are not limited to: Design and optimization of genetic systems Genetic circuit design and their principles for their organization into programs Computational methods to aid the design of genetic systems Experimental methods to quantify genetic parts, circuits, and metabolic fluxes Genetic parts libraries: their creation, analysis, and ontological representation Protein engineering including computational design Metabolic engineering and cellular manufacturing, including biomass conversion Natural product access, engineering, and production Creative and innovative applications of cellular programming Medical applications, tissue engineering, and the programming of therapeutic cells Minimal cell design and construction Genomics and genome replacement strategies Viral engineering Automated and robotic assembly platforms for synthetic biology DNA synthesis methodologies Metagenomics and synthetic metagenomic analysis Bioinformatics applied to gene discovery, chemoinformatics, and pathway construction Gene optimization Methods for genome-scale measurements of transcription and metabolomics Systems biology and methods to integrate multiple data sources in vitro and cell-free synthetic biology and molecular programming Nucleic acid engineering.