Mitigating Night Biomass Loss in Outdoor Pilot-Scale Mixotrophic Algal Cultivation of Monoraphidium minutum Using Flue Gas Condensate and Cheese Whey.

IF 3.5 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology and Bioengineering Pub Date : 2025-07-18 DOI:10.1002/bit.70027

Quyen Nham,Tristan Gordon,Hanna Farnelid,Catherine Legrand,Elin Lindehoff

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Abstract

In algal cultivation, nighttime biomass loss due to respiration and cell mortality can considerably reduce the amount of biomass produced during daylight. The adverse effect can be counteracted by mixotrophic cultivation, where an organic carbon (OC) source is used to supply the energy required for cell maintenance and division during darkness. The potential for mixotrophic cultivation to mitigate night biomass loss has yet to be tested under outdoor, large-scale conditions that use raw industrial waste streams, particularly during low-light seasons. We investigated night biomass loss in cultivation of the strain Monoraphidium minutum KAC90 in outdoor 1 m3 raceway ponds during the Nordic autumn. Flue gas condensate (nitrogen source) and cheese whey (phosphorus and OC source) were used for the mixotrophic treatment, while potassium monophosphate (phosphorus source) was used for the photoautotrophic control. Results indicate that under high OC availability, the mixotrophic treatment had a night biomass gain of 33% ± 16%, whereas it experienced a night biomass loss of 10% ± 9% under low OC. In contrast, the photoautotrophic control showed a night biomass loss of 5% ± 15%. In the mixotrophic treatment, algal biomass had a higher carbohydrate content, but lower levels of lipids and proteins than the photoautotrophic cultures. The cultivation of algae using cheese whey may increase biomass accumulation in darkness, enhancing the overall production of algal biomass rich in carbohydrates.

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利用烟气冷凝液和奶酪乳清减少室外混合营养藻类培养中夜生物量损失

在藻类培养中，由于呼吸作用和细胞死亡造成的夜间生物量损失可以大大减少白天产生的生物量。这种不利影响可以通过混合营养培养来抵消，其中有机碳（OC）源用于在黑暗中提供细胞维持和分裂所需的能量。混合营养栽培减轻夜间生物量损失的潜力尚未在室外大规模条件下进行测试，这些条件使用原始工业废物流，特别是在光照不足的季节。研究了北欧秋季在室外1 m3沟道池中栽培单芽孢菌（Monoraphidium minutum KAC90）夜间生物量损失。采用烟气冷凝液（氮源）和干酪乳清（磷源和OC源）进行混合营养处理，单磷酸钾（磷源）进行光自养控制。结果表明，在高OC有效度下，混合营养处理夜间生物量增加33%±16%，而在低OC有效度下，混合营养处理夜间生物量减少10%±9%。相比之下，光自养对照夜间生物量损失为5%±15%。在混合营养处理中，藻类生物量的碳水化合物含量高于光自养培养，但脂质和蛋白质含量低于光自养培养。利用干酪乳清培养藻类可以增加黑暗中生物量的积累，提高富含碳水化合物的藻类生物量的整体产量。

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来源期刊

Biotechnology and Bioengineering 工程技术-生物工程与应用微生物

CiteScore

7.90

自引率

5.30%

发文量

280

审稿时长

2.1 months

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