Synthesis of magnetic N-doped carbon dots as pH-responsive targeted molecule cargo and its antioxidant and antibacterial behaviour.

IF 2.1 4区 医学 Q3 PHARMACOLOGY & PHARMACY
Hayat Alzahrani, Mohammed S Alkaltham, Tawfiq Alsulami, Abdulhakeem Alzahrani, Suleiman A Althawab
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引用次数: 0

Abstract

This study successfully generated magnetic N-doped carbon dots (CDs-MNPs) that exhibit two distinct functions: pH-responsive targeted drug delivery and powerful antioxidant action. The structural integrity, magnetic characteristics, and thermal stability of the samples were confirmed using comprehensive characterisation techniques, such as scanning electron microscopy, superconducting quantum interference device, Fourier Transform Infrared Spectroscopy, X-ray diffraction, continuous-wave electron para-magnetic resonance, X-ray photoelectron spectroscopy, surface porosity and thermogravimetric analysis. The CDs-MNPs displayed pH-dependent drug release profiles that conformed to zero-order, Higuchi, and Peppas models, demonstrating their ability to provide regulated release. The antioxidant activity of the carbon dots was assessed using the DPPH assay, where the radical scavenging capacity exceeded 80 %. This high level of activity was attributed to the synergistic effects of nitrogen doping and the functional groups present on the carbon dots. The biocompatibility of the specimen (up to 100 mg mL-1), which is essential for biomedical applications, was confirmed by MTT assays. This study highlights the potential of CDs-MNPs as an effective option for therapeutic interventions, providing customised drug delivery and antioxidant advantages. The antibacterial activity of CDs-MNPs was evaluated against Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus bacterial strains, demonstrating significant efficacy. These results highlight the potential of CD-based nanobactericides for applications in biomedical and food monitoring.

磁性n掺杂碳点作为ph响应靶向分子货物的合成及其抗氧化和抗菌性能。
该研究成功地生成了磁性n掺杂碳点(cd - mnps),具有两种不同的功能:ph响应性靶向药物传递和强大的抗氧化作用。利用扫描电镜、超导量子干涉仪、傅里叶变换红外光谱、x射线衍射、连续波电子准磁共振、x射线光电子能谱、表面孔隙率和热重分析等综合表征技术,对样品的结构完整性、磁性和热稳定性进行了验证。CDs-MNPs显示ph依赖的药物释放谱符合零阶、Higuchi和Peppas模型,证明了它们提供调节释放的能力。采用DPPH法测定碳点的抗氧化活性,其自由基清除能力超过80%。这种高活性归因于氮掺杂和碳点上的官能团的协同作用。样品的生物相容性(高达100 mg mL-1)对生物医学应用至关重要,经MTT测定证实。这项研究强调了cd - mnps作为治疗干预的有效选择的潜力,提供了定制的药物输送和抗氧化优势。对CDs-MNPs对革兰氏阴性大肠杆菌和革兰氏阳性金黄色葡萄球菌的抑菌活性进行了评价,显示出显著的抑菌效果。这些结果突出了基于cd的纳米杀菌剂在生物医学和食品监测方面的应用潜力。
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来源期刊
Acta Pharmaceutica
Acta Pharmaceutica PHARMACOLOGY & PHARMACY-
CiteScore
5.20
自引率
3.60%
发文量
20
审稿时长
>12 weeks
期刊介绍: AP is an international, multidisciplinary journal devoted to pharmaceutical and allied sciences and contains articles predominantly on core biomedical and health subjects. The aim of AP is to increase the impact of pharmaceutical research in academia, industry and laboratories. With strong emphasis on quality and originality, AP publishes reports from the discovery of a drug up to clinical practice. Topics covered are: analytics, biochemistry, biopharmaceutics, biotechnology, cell biology, cell cultures, clinical pharmacy, drug design, drug delivery, drug disposition, drug stability, gene technology, medicine (including diagnostics and therapy), medicinal chemistry, metabolism, molecular modeling, pharmacology (clinical and animal), peptide and protein chemistry, pharmacognosy, pharmacoepidemiology, pharmacoeconomics, pharmacodynamics and pharmacokinetics, protein design, radiopharmaceuticals, and toxicology.
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