Stimulating effect of copper ions and copper nanoparticles on a nitrifying sludge process: microrespirometric and physicochemical assessment

IF 2.4 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of chemical technology and biotechnology Pub Date : 2025-05-27 DOI:10.1002/jctb.7904

Gabriel R. Hernandez-Martinez, Emma O. Fuentes-Ramírez, Cherif Ben Youssef, Juan E. Ruiz Espinoza, Alejandro Zepeda

{"title":"Stimulating effect of copper ions and copper nanoparticles on a nitrifying sludge process: microrespirometric and physicochemical assessment","authors":"Gabriel R. Hernandez-Martinez, Emma O. Fuentes-Ramírez, Cherif Ben Youssef, Juan E. Ruiz Espinoza, Alejandro Zepeda","doi":"10.1002/jctb.7904","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> BACKGROUND</h3>\n \n <p>Copper nanoparticles are used at an accelerated rate in several sectors, generating concern about their impact on human health and the environment. In this study, we characterized the physicochemical behavior and physiological effects of copper ions (Cu(II) ions) and copper nanoparticles (CuNPs) on a nitrifying sludge consortium.</p>\n </section>\n \n <section>\n \n <h3> RESULTS</h3>\n \n <p>Both Cu(II) ions and CuNPs exerted a hormetic effect, stimulating nitrifying sludge biomass. Stimulation was quantified by the percentage of enhancement (<i>E</i><sub><i>OUR</i></sub>), maximum specific oxygen uptake rate (<i>OUR</i><sub><i>max</i></sub>), and substrate affinity constant (<i>K</i><sub><i>S</i></sub>). The results showed that, within the range of 0–20 mg L<sup>−1</sup>, both Cu(II) ions and CuNPs stimulated the nitrifying respiratory process, with maximum stimulation effects of 82.8% and 61.1% at 5 mg L<sup>−1</sup>, respectively. Both Cu(II) ions and CuNPs only increased the <i>OUR</i><sub><i>max</i></sub>, but not the <i>K</i><sub><i>S</i></sub>.</p>\n </section>\n \n <section>\n \n <h3> CONCLUSION</h3>\n \n <p>These findings indicate that copper deficiency could limit nitrogen removal in wastewater treatment. Furthermore, physicochemical characterization allowed us to determine that the observed stimulating effect may be caused by different factors, such as the aggregation, speciation, and adsorption of copper species in the nitrifying sludge consortium. © 2025 Society of Chemical Industry (SCI).</p>\n </section>\n </div>","PeriodicalId":15335,"journal":{"name":"Journal of chemical technology and biotechnology","volume":"100 8","pages":"1716-1723"},"PeriodicalIF":2.4000,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of chemical technology and biotechnology","FirstCategoryId":"5","ListUrlMain":"https://scijournals.onlinelibrary.wiley.com/doi/10.1002/jctb.7904","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

BACKGROUND

Copper nanoparticles are used at an accelerated rate in several sectors, generating concern about their impact on human health and the environment. In this study, we characterized the physicochemical behavior and physiological effects of copper ions (Cu(II) ions) and copper nanoparticles (CuNPs) on a nitrifying sludge consortium.

RESULTS

Both Cu(II) ions and CuNPs exerted a hormetic effect, stimulating nitrifying sludge biomass. Stimulation was quantified by the percentage of enhancement (E_OUR), maximum specific oxygen uptake rate (OUR_max), and substrate affinity constant (K_S). The results showed that, within the range of 0–20 mg L⁻¹, both Cu(II) ions and CuNPs stimulated the nitrifying respiratory process, with maximum stimulation effects of 82.8% and 61.1% at 5 mg L⁻¹, respectively. Both Cu(II) ions and CuNPs only increased the OUR_max, but not the K_S.

CONCLUSION

These findings indicate that copper deficiency could limit nitrogen removal in wastewater treatment. Furthermore, physicochemical characterization allowed us to determine that the observed stimulating effect may be caused by different factors, such as the aggregation, speciation, and adsorption of copper species in the nitrifying sludge consortium. © 2025 Society of Chemical Industry (SCI).

Abstract Image

查看原文本刊更多论文

铜离子和铜纳米颗粒对硝化污泥过程的刺激作用：微呼吸和理化评价

背景：铜纳米颗粒在若干部门的使用速度加快，引起人们对其对人类健康和环境影响的关注。在这项研究中，我们表征了铜离子（Cu（II）离子）和铜纳米粒子（Cu纳米粒子）在硝化污泥中的物理化学行为和生理效应。结果Cu（II）离子和CuNPs均能激发硝化污泥生物量。通过增强百分比（EOUR）、最大比摄氧量（OURmax）和底物亲和常数（KS）来量化刺激。结果表明，在0 ~ 20 mg L−1范围内，Cu（II）离子和CuNPs均对硝化呼吸过程有刺激作用，在5 mg L−1时的刺激效果最大，分别为82.8%和61.1%。Cu（II）离子和Cu（II）离子只增加了OURmax，而对KS没有影响。结论缺铜会限制污水处理过程中氮的去除。此外，物理化学表征使我们能够确定所观察到的刺激效应可能是由不同因素引起的，例如硝化污泥财团中铜物种的聚集，形成和吸附。©2025化学工业学会（SCI）。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of chemical technology and biotechnology 工程技术-工程：化工

CiteScore

7.00

自引率

5.90%

发文量

268

审稿时长

1.7 months

期刊介绍： Journal of Chemical Technology and Biotechnology(JCTB) is an international, inter-disciplinary peer-reviewed journal concerned with the application of scientific discoveries and advancements in chemical and biological technology that aim towards economically and environmentally sustainable industrial processes.