Gökhan Arkan, Betül Öğüt, Aylar Poyraz, Yusuf Hakan Çavuşoğlu
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引用次数: 0
Abstract
Small bowel volvulus is a clinical condition that causes intestinal ischemia-reperfusion (I/R) injury, leading to severe tissue damage and high mortality rates. Polydatin, a natural stilbenoid polyphenol, has demonstrated anti-inflammatory and antioxidant properties. This study aimed to evaluate the protective effects of polydatin on I/R injury using an experimental small bowel volvulus model.A total of 24 healthy female Wistar albino rats were divided into four groups: Sham (Group 1), Polydatin (Group 2), I/R (Group 3), and Treatment (Group 4). In Group 1, no I/R procedure was applied, and intraperitoneal saline was administered. Group 2 received 20 mg/kg intraperitoneal polydatin without I/R. In Group 3, a 5-cm segment of the small intestine was twisted 360 degrees clockwise, leading to 2 hours of ischemia and 2 hours of reperfusion. Group 4 received 20 mg/kg intraperitoneal polydatin before reperfusion. Blood and intestinal tissue samples were collected for biochemical and histopathological analysis.Serum total antioxidant status (TAS) levels were significantly higher in the treatment group compared with the I/R group (p = 0.004). Serum total oxidant status (TOS) levels were significantly elevated in the I/R group compared with all other groups (p < 0.001) but were significantly reduced in the treatment group (p < 0.001). Tissue oxidative stress index (OSI) values were significantly lower in the treatment group compared with the I/R group (p = 0.004). Although serum OSI levels and tissue TAS and TOS values showed a favorable trend, they were not statistically significant. Histopathological evaluations revealed a marked reduction in tissue damage in the treatment group compared with the I/R group.Polydatin exerts protective effects against I/R injury in an experimental small bowel volvulus model by reducing oxidative stress and histopathological damage. These findings highlight its therapeutic potential and warrant further clinical research.
小肠扭转是一种引起肠缺血再灌注(I/R)损伤的临床疾病,可导致严重的组织损伤和高死亡率。聚蝶苷是一种天然的二苯乙烯类多酚,具有抗炎和抗氧化的特性。本研究旨在通过实验性小肠扭转模型,评估多丹素对I/R损伤的保护作用。将24只健康雌性Wistar白化大鼠分为4组:Sham组(1组)、Polydatin组(2组)、I/R组(3组)、治疗组(4组)。第1组不进行I/R手术,并腹腔注射生理盐水。2组患者腹腔注射聚胆苷20 mg/kg,无I/R。第3组将小肠5cm段顺时针旋转360度,缺血2小时,再灌注2小时。4组大鼠再灌注前腹腔注射聚肽20 mg/kg。采集血液和肠道组织标本进行生化和组织病理学分析。治疗组血清总抗氧化状态(TAS)水平显著高于I/R组(p = 0.004)。与其他各组相比,I/R组血清总氧化状态(TOS)水平显著升高(p p p = 0.004)。血清OSI水平和组织TAS、TOS值虽呈良好趋势,但无统计学意义。组织病理学评估显示,与I/R组相比,治疗组的组织损伤明显减少。多丹素通过减少氧化应激和组织病理学损伤,对实验性小肠扭转模型I/R损伤具有保护作用。这些发现突出了其治疗潜力,值得进一步的临床研究。
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