Correlation and predictive modeling of serum exosomal miRNAs and serological biomarkers for lymph node metastasis in gastric cancer.

IF 2.9 3区 医学 Q2 ONCOLOGY
American journal of cancer research Pub Date : 2025-06-15 eCollection Date: 2025-01-01 DOI:10.62347/AOJE4206
Quandong Li, Fang Nie, Dezhi Huang, Yongping Lin, Junjie Wan
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引用次数: 0

Abstract

Objective: To evaluate the predictive potential of serum exosomal microRNAs (miRNAs) and traditional serological biomarkers for lymph node metastasis in gastric cancer and to assess their applicability in clinical practice.

Methods: This retrospective study included 845 gastric cancer patients treated between January 2020 and December 2023, as the training cohort. Patients were stratified into lymph node-positive (n = 231) and lymph node-negative (n = 614) groups based on postoperative pathological evaluation. Serum exosomal miRNAs and conventional serological biomarkers were quantified and compared between groups. Multivariate logistic regression analysis was conducted to identify independent predictors. Model performance was validated using an independent test cohort comprising 277 patients (74 lymph node-positive, 203 lymph node-negative).

Results: Patients with lymph node metastasis exhibited significantly elevated expression of miR-21, miR-20a, miR-27a, and miR-106a. Serological markers that were significantly higher in the lymph node positive group included carbohydrate antigen 724, carcinoembryonic antigen, hepatocyte growth factor, vascular endothelial growth factor, interleukin-6, and circulating cell-free DNA (all P < 0.05). A combined predictive model integrating both miRNA and serological data demonstrated strong diagnostic performance, with an area under the curve of 0.816 in the training cohort and 0.817 in the validation cohort.

Conclusion: Serum exosomal miRNAs and serological biomarkers are significantly associated with lymph node metastasis in gastric cancer.

胃癌淋巴结转移的血清外泌体mirna和血清学生物标志物的相关性和预测模型。
目的:评价血清外泌体microRNAs (miRNAs)和传统血清学标志物对胃癌淋巴结转移的预测潜力,并评价其在临床中的适用性。方法:本回顾性研究纳入2020年1月至2023年12月期间接受治疗的845例胃癌患者作为训练队列。根据术后病理评价将患者分为淋巴结阳性组(n = 231)和淋巴结阴性组(n = 614)。对血清外泌体mirna和常规血清学生物标志物进行量化比较。进行多变量logistic回归分析以确定独立预测因子。通过由277例患者(74例淋巴结阳性,203例淋巴结阴性)组成的独立测试队列验证了模型的性能。结果:淋巴结转移患者miR-21、miR-20a、miR-27a和miR-106a的表达显著升高。淋巴结阳性组血清学指标碳水化合物抗原724、癌胚抗原、肝细胞生长因子、血管内皮生长因子、白细胞介素-6、循环游离DNA均显著升高(P < 0.05)。结合miRNA和血清学数据的联合预测模型显示出较强的诊断性能,训练组和验证组的曲线下面积分别为0.816和0.817。结论:血清外泌体mirna和血清学标志物与胃癌淋巴结转移有显著相关性。
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来源期刊
自引率
3.80%
发文量
263
期刊介绍: The American Journal of Cancer Research (AJCR) (ISSN 2156-6976), is an independent open access, online only journal to facilitate rapid dissemination of novel discoveries in basic science and treatment of cancer. It was founded by a group of scientists for cancer research and clinical academic oncologists from around the world, who are devoted to the promotion and advancement of our understanding of the cancer and its treatment. The scope of AJCR is intended to encompass that of multi-disciplinary researchers from any scientific discipline where the primary focus of the research is to increase and integrate knowledge about etiology and molecular mechanisms of carcinogenesis with the ultimate aim of advancing the cure and prevention of this increasingly devastating disease. To achieve these aims AJCR will publish review articles, original articles and new techniques in cancer research and therapy. It will also publish hypothesis, case reports and letter to the editor. Unlike most other open access online journals, AJCR will keep most of the traditional features of paper print that we are all familiar with, such as continuous volume, issue numbers, as well as continuous page numbers to retain our comfortable familiarity towards an academic journal.
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