Increased levels of tumor necrosis factor-alpha in rat with traumatic brain injury after NeuroAid (MLC 901) administration.

Surgical neurology international Pub Date : 2025-06-27 eCollection Date: 2025-01-01 DOI:10.25259/SNI_301_2025

Rohadi, Bambang Priyanto, Andi Asadul Islam, Mochammad Hatta, Agussalim Bukhari, Rozikin, I Wayan Gede Artawan Eka Putra, Lalu Muhammad Abdurrosid, Krisna Tsaniadi Prihastomo

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Abstract

Background: Tumor necrosis factor-alpha (TNF-α) is an inflammatory cytokine produced by macrophages in acute inflammatory processes and plays roles in cell signaling that cause necrosis and apoptosis. This study aimed to show whether there was an effect of Neuroaid (MLC 901) on TNF-α levels in rats with traumatic brain injury (TBI) measured using the Enzyme-linked immunosorbent assay in the peripheral blood.

Methods: A total of 10 Sprague-Dawley rats were divided into two groups, one group was given MLC 901 (n = 5), and the other group was not given MLC 901 (NaCl 0.9%) (n = 5). All groups were treated with brain injury using the modified Marmarou model. The measurements of TNF-α were performed at 30 min and 6 weeks after brain injury.

Results: At 30 min after brain injury, the TNF-α level in the MLC 901 group was higher (3564.8) than the 0.9% NaCl group (3453.6), but it was not statistically significant (P = 0.830). At 6 weeks of treatment, the TNF-α level in the MLC 901 group (2576.6) was higher than the 0.9% NaCl group (1383.4) and statistically significant (P = 0.001). This study showed that the administration of MLC 901 could increase TNF-α levels at 6 weeks after treatment.

Conclusion: MLC 901 increases TNF-α levels in rats with TBI, with a significant rise observed at 6 weeks, suggesting a sustained inflammatory response.

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神经援助（MLC 901）给药后创伤性脑损伤大鼠肿瘤坏死因子- α水平升高

背景：肿瘤坏死因子-α (Tumor necrosis factor -α, TNF-α)是巨噬细胞在急性炎症过程中产生的一种炎性细胞因子，在引起坏死和凋亡的细胞信号传导中起作用。本研究旨在表明Neuroaid （MLC 901）是否对外伤性脑损伤（TBI）大鼠外周血中TNF-α水平的影响，采用酶联免疫吸附法测定。方法：将10只Sprague-Dawley大鼠分为两组，一组给予MLC 901 (n = 5)，另一组不给予MLC 901 (NaCl 0.9%) （n = 5）。各组均采用改良Marmarou模型进行脑损伤治疗。分别于脑损伤后30分钟和6周测定TNF-α。结果：脑损伤后30min， MLC 901组TNF-α水平（3564.8）高于0.9% NaCl组（3453.6），但差异无统计学意义（P = 0.830）。治疗6周时，MLC 901组TNF-α水平（2576.6）高于0.9% NaCl组（1383.4），差异有统计学意义（P = 0.001）。本研究表明，在治疗后6周，给予MLC 901可增加TNF-α水平。结论：MLC 901增加TBI大鼠TNF-α水平，6周显著升高，提示持续炎症反应。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Surgical neurology international

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