{"title":"cth-2/mpst-1-dependent H2S deficiency enhances acrylonitrile acute toxicity in Caenorhabditis elegans","authors":"Bobo Yang , Michael Aschner , Rongzhu Lu","doi":"10.1016/j.neuro.2025.07.002","DOIUrl":null,"url":null,"abstract":"<div><div>Acrylonitrile (AN) is a toxic, colorless to pale-yellow liquid extensively used in industrial production and has been linked to neurotoxicity. Though our previous study showed a correlation between AN-induced neurotoxicity and gasotransmitter hydrogen sulfide (H<sub>2</sub>S) in mammalian cells, experimental evidence on overall animal toxicity and specific neurological injury is still limited. We aimed to further explore the molecular association between H<sub>2</sub>S and AN-induced acute toxicity in <em>Caenorhabditis elegans (C. elegans)</em> by using its genetic advantages, and provide experimental evidence for the validation of H<sub>2</sub>S donors as AN antidote. In the present study, we demonstrated that acute AN exposure resulted in toxicity as evidenced by changes in death rate, locomotor behavior, brood size, dopaminergic neuron morphology, and oxidative stress. Notably, AN inhibited the H<sub>2</sub>S content, which was double-examined by methylene blue spectrophotometry and lead acetate paper assay. Furthermore, AN significantly decreased 3-mercaptopyruvate sulphurtransferase (3-MPST)-mediated H<sub>2</sub>S synthesizing activity and the transcription level of the corresponding coding gene <em>mpst-1</em> but had no effect on the cystathionine β synthetase (CBS)/cystathionine γ lyase (CSE)-mediated H<sub>2</sub>S synthesizing activity using L-cysteine as a common substrate and the mRNA levels of H<sub>2</sub>S oxidative metabolism enzymes. <em>cth-2</em> and <em>mpst-1</em> mutations significantly downregulated the H<sub>2</sub>S content and the corresponding H<sub>2</sub>S synthesizing activity, and further enhanced the AN-induced toxicity response including lethality, brood size and lifespan. In contrast, H<sub>2</sub>S donor GYY4137 significantly attenuated the AN-damaged survival rate, body bends, and dopaminergic neuron morphology. Our findings demonstrated that the reduction of H<sub>2</sub>S mediates the acute toxicity of AN.</div></div>","PeriodicalId":19189,"journal":{"name":"Neurotoxicology","volume":"110 ","pages":"Pages 64-73"},"PeriodicalIF":3.4000,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Neurotoxicology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0161813X25000865","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"NEUROSCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Acrylonitrile (AN) is a toxic, colorless to pale-yellow liquid extensively used in industrial production and has been linked to neurotoxicity. Though our previous study showed a correlation between AN-induced neurotoxicity and gasotransmitter hydrogen sulfide (H2S) in mammalian cells, experimental evidence on overall animal toxicity and specific neurological injury is still limited. We aimed to further explore the molecular association between H2S and AN-induced acute toxicity in Caenorhabditis elegans (C. elegans) by using its genetic advantages, and provide experimental evidence for the validation of H2S donors as AN antidote. In the present study, we demonstrated that acute AN exposure resulted in toxicity as evidenced by changes in death rate, locomotor behavior, brood size, dopaminergic neuron morphology, and oxidative stress. Notably, AN inhibited the H2S content, which was double-examined by methylene blue spectrophotometry and lead acetate paper assay. Furthermore, AN significantly decreased 3-mercaptopyruvate sulphurtransferase (3-MPST)-mediated H2S synthesizing activity and the transcription level of the corresponding coding gene mpst-1 but had no effect on the cystathionine β synthetase (CBS)/cystathionine γ lyase (CSE)-mediated H2S synthesizing activity using L-cysteine as a common substrate and the mRNA levels of H2S oxidative metabolism enzymes. cth-2 and mpst-1 mutations significantly downregulated the H2S content and the corresponding H2S synthesizing activity, and further enhanced the AN-induced toxicity response including lethality, brood size and lifespan. In contrast, H2S donor GYY4137 significantly attenuated the AN-damaged survival rate, body bends, and dopaminergic neuron morphology. Our findings demonstrated that the reduction of H2S mediates the acute toxicity of AN.
期刊介绍:
NeuroToxicology specializes in publishing the best peer-reviewed original research papers dealing with the effects of toxic substances on the nervous system of humans and experimental animals of all ages. The Journal emphasizes papers dealing with the neurotoxic effects of environmentally significant chemical hazards, manufactured drugs and naturally occurring compounds.