Effects of Chronic Photobiomodulation with Transcranial Near-Infrared Laser on Seizure Frequency and Brain Metabolomics of Rats with Pilocarpine-Induced Seizures.

IF 4.3 2区 医学 Q1 NEUROSCIENCES
Molecular Neurobiology Pub Date : 2025-11-01 Epub Date: 2025-07-14 DOI:10.1007/s12035-025-05175-y
Fabrizio Dos Santos Cardoso, Ricardo Mario Arida, Eduardo Alves da Silva, Ana Carolina Ribeiro de Oliveira, Wagner Ferreira Dos Santos, Rodrigo Álvaro Brandão Lopes-Martins, Francisco Gonzalez-Lima, Norberto Cysne Coimbra, Sérgio Gomes da Silva
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引用次数: 0

Abstract

Epilepsy is a chronic brain disorder characterized by abnormal and highly synchronous activity of neuronal cells. This condition is often associated with neuronal hyperexcitability and metabolic imbalances in regions such as the neocortex and archicortex (hippocampus). Pharmacological treatment with antiepileptic drugs has been essential in most cases. However, some patients continue to experience seizures despite medication. Consequently, the search for alternative treatments and new therapeutic interventions has garnered significant interest within the medical community. Among these, photobiomodulation (PBM) shows great promise, particularly due to its cerebral and neuroprotective effects. We evaluated and compared the seizure frequency and the neocortical and hippocampal metabolomic profiles of pilocarpine-induced epileptic rats exposed to chronic transcranial photobiomodulation treatment (30 days of treatment) with an 810-nm, 100-mW laser. Our data show that PBM treatment significantly reduced the seizure frequency in rats with pilocarpine-induced seizures. Additionally, significant changes were observed in the metabolomic profiles of the neocortex and hippocampus. In the neocortex, the treatment reduced metabolic pathways associated with excitotoxicity and oxidative stress. In the hippocampus, an increase in phenylalanine concentration was detected. In addition, the reduction in the number of seizures in laser-treated rats with pilocarpine-induced seizures was correlated with lower neocortical lysine concentration. Taken together, our findings indicate that transcranial PBM prevents the increase in seizure frequency in rats with epilepsy and modulates the brain metabolic pathways of epileptic rats.

经颅近红外激光慢性光生物调节对匹罗卡品诱发大鼠癫痫发作频率和脑代谢组学的影响。
癫痫是一种以神经细胞异常和高度同步活动为特征的慢性脑部疾病。这种情况通常与神经兴奋性亢进和新皮质和皮质(海马)等区域的代谢失衡有关。在大多数情况下,抗癫痫药物的药理治疗是必不可少的。然而,尽管有药物治疗,一些患者仍会出现癫痫发作。因此,寻找替代疗法和新的治疗干预措施在医学界引起了极大的兴趣。其中,光生物调节(PBM)显示出巨大的前景,特别是由于其大脑和神经保护作用。我们评估并比较了皮罗卡品诱导的癫痫大鼠接受810 nm、100 mw激光慢性经颅光生物调节治疗(治疗30天)后的癫痫发作频率、新皮质和海马代谢组学特征。我们的数据显示,PBM治疗显著降低了匹罗卡品诱发癫痫发作的大鼠的癫痫发作频率。此外,新皮层和海马的代谢组学特征也发生了显著变化。在新皮层中,治疗减少了与兴奋毒性和氧化应激相关的代谢途径。海马区苯丙氨酸浓度升高。此外,激光治疗的匹罗卡品诱发癫痫大鼠癫痫发作次数的减少与较低的新皮质赖氨酸浓度有关。综上所述,我们的研究结果表明,经颅PBM可以防止癫痫大鼠癫痫发作频率的增加,并调节癫痫大鼠的脑代谢途径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Molecular Neurobiology
Molecular Neurobiology 医学-神经科学
CiteScore
9.00
自引率
2.00%
发文量
480
审稿时长
1 months
期刊介绍: Molecular Neurobiology is an exciting journal for neuroscientists needing to stay in close touch with progress at the forefront of molecular brain research today. It is an especially important periodical for graduate students and "postdocs," specifically designed to synthesize and critically assess research trends for all neuroscientists hoping to stay active at the cutting edge of this dramatically developing area. This journal has proven to be crucial in departmental libraries, serving as essential reading for every committed neuroscientist who is striving to keep abreast of all rapid developments in a forefront field. Most recent significant advances in experimental and clinical neuroscience have been occurring at the molecular level. Until now, there has been no journal devoted to looking closely at this fragmented literature in a critical, coherent fashion. Each submission is thoroughly analyzed by scientists and clinicians internationally renowned for their special competence in the areas treated.
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