Methanol leaf extract of Azadirachta indica mitigates isoproterenol-induced myocardial infarction through the modulation of oxidative stress, and PPARα and BCL2 signaling in rats.

IF 1.9 Q3 CHEMISTRY, MEDICINAL

Avicenna Journal of Phytomedicine Pub Date : 2025-07-01 DOI:10.22038/ajp.2024.25277

Amirah Folashade Yusuf, Temitayo Olabisi Ajibade, Oluwaseun Olarenwaju Esan, Racheal Ebunoluwa Asenuga, Moyinoluwa Onoja, Matthew Obot Akpan, Joseph Ayotunde Badejo, Temidayo Olutayo Omobowale, Ademola Adetokunbo Oyagbemi, Adeolu Alex Adedapo, Oluwafemi Omoniyi Oguntibeju, Momoh Audu Yakubu

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Abstract

Objective: Evaluation of Azadirachta indica's potential on the modulation of blood pressure parameters, antioxidant defense status, as well as immunohistochemical expressions of Peroxisome proliferator-activated receptor α (PPARα) and Bcl-2 (B-cell lymphoma 2) in rats exposed to isoproterenol was the objective of this study.

Materials and methods: Fifty rats (Rattus norvegicus) of the Wistar strain were used, with myocardial infarction induced by intraperitoneal administration of isoproterenol (ISO) for two consecutive days. Cardiac and renal biomarkers of oxidative stress, blood pressure parameters, electrocardiography, and immunohistochemical staining of PPARα and BCL2 were performed.

Results: ISO toxicity heightened blood pressure parameters, aggravated oxidative processes, declined antioxidant defense system, and decreased immunohistochemical expressions of PPARα and BCL2. Interestingly, A. indica improved antioxidant status, lowered free radical generation, mitigated serum myeloperoxidase and xanthine oxidase activities, respectively.

Conclusion: Mitigation of oxidative mechanisms and antihypertensive effects of Azadirachta indica suggest a positive modulatory role for the medicinal plant in isoproterenol-induced myocardial infarction.

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印楝甲醇叶提取物通过调节氧化应激、PPARα和BCL2信号通路减轻异丙肾上腺素诱导的大鼠心肌梗死。

目的：探讨印印果对异丙肾上腺素暴露大鼠血压参数、抗氧化防御状态以及过氧化物酶体增殖物激活受体α (PPARα)和b细胞淋巴瘤2 （Bcl-2）免疫组织化学表达的调节作用。材料与方法：选用Wistar品系褐家鼠50只，连续2 d腹腔注射异丙肾上腺素（ISO）诱导心肌梗死。进行心脏和肾脏氧化应激生物标志物、血压参数、心电图以及PPARα和BCL2的免疫组织化学染色。结果：ISO毒性使血压参数升高，氧化过程加剧，抗氧化防御系统下降，PPARα和BCL2的免疫组织化学表达降低。有趣的是，籼稻改善了抗氧化状态，降低了自由基的产生，分别减轻了血清髓过氧化物酶和黄嘌呤氧化酶的活性。结论：印楝在异丙肾上腺素诱导的心肌梗死中具有抗氧化机制和降压作用。

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