Scaling-up the Bioconversion of Lignin to 2,4-Pyridinedicarboxylic Acid With Engineered Pseudomonas putida for Bio-Based Plastics Production.

IF 3.5 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Jan Seeger,Susanne Müller,Helena Gómez-Álvarez,Goran M M Rashid,Timothy D H Bugg,Eduardo Díaz,Ralf Takors
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引用次数: 0

Abstract

2,4-pyridinedicarboxylic acid (PDCA) is a promising bio-based compound to substitute petroleum-derived terephthalic acid in plastics. It is produced through the microbial conversion of lignin substrates with engineered microorganisms like Pseudomonas putida. To this point, an efficient bioproduction process for PDCA has not yet been established. In this study, we optimized PDCA production with engineered P. putida ligAB and demonstrated bioproduction at up to 30 L scale. PDCA was produced with a volumetric productivity of 390 mg/L/h from the precursor protocatechuate and the product titer was doubled compared to previously reported work. Lignin feedstock and pretreatment combinations were screened to access lignin as substrate for PDCA production. Sodium hydroxide lignin with alkali + heat pretreatment yielded 33 mg/L PDCA at a production rate of 0.1 mg/g/h. Low PDCA production rates could be overcome by developing a bacterial mixed culture by adding the engineered strain Rhodococcus jostii ΔpcaHG that supplies PCA from lignin degradation. The mixed culture increased PDCA productivity of P. putida ligAB by factor 19 (1.9 mg/gP. putida/h).
利用工程恶臭假单胞菌扩大木质素转化为2,4-吡啶二羧酸的生物基塑料生产。
2,4-吡啶二羧酸(PDCA)是一种很有希望取代石油衍生对苯二甲酸在塑料中的应用的生物基化合物。它是通过工程微生物如恶臭假单胞菌对木质素底物的微生物转化而产生的。到目前为止,PDCA的高效生物生产工艺尚未建立。在这项研究中,我们优化了用工程化的p.p . putida ligAB生产PDCA,并在30 L的规模上进行了生物生产。从前体原儿茶酸中生产PDCA的体积产率为390 mg/L/h,产品滴度比先前报道的工作提高了一倍。筛选了木质素原料和预处理组合,获得了木质素作为PDCA生产的底物。碱+热预处理的氢氧化钠木质素在0.1 mg/g/h的产率下可得33 mg/L的PDCA。低PDCA的生产速度可以通过发展细菌混合培养,加入工程菌株乔氏红球菌ΔpcaHG,提供PCA从木质素降解。混合培养使恶臭p.p . putida ligAB的PDCA产量提高了19倍(1.9 mg/gP)。putida / h)。
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来源期刊
Biotechnology and Bioengineering
Biotechnology and Bioengineering 工程技术-生物工程与应用微生物
CiteScore
7.90
自引率
5.30%
发文量
280
审稿时长
2.1 months
期刊介绍: Biotechnology & Bioengineering publishes Perspectives, Articles, Reviews, Mini-Reviews, and Communications to the Editor that embrace all aspects of biotechnology. These include: -Enzyme systems and their applications, including enzyme reactors, purification, and applied aspects of protein engineering -Animal-cell biotechnology, including media development -Applied aspects of cellular physiology, metabolism, and energetics -Biocatalysis and applied enzymology, including enzyme reactors, protein engineering, and nanobiotechnology -Biothermodynamics -Biofuels, including biomass and renewable resource engineering -Biomaterials, including delivery systems and materials for tissue engineering -Bioprocess engineering, including kinetics and modeling of biological systems, transport phenomena in bioreactors, bioreactor design, monitoring, and control -Biosensors and instrumentation -Computational and systems biology, including bioinformatics and genomic/proteomic studies -Environmental biotechnology, including biofilms, algal systems, and bioremediation -Metabolic and cellular engineering -Plant-cell biotechnology -Spectroscopic and other analytical techniques for biotechnological applications -Synthetic biology -Tissue engineering, stem-cell bioengineering, regenerative medicine, gene therapy and delivery systems The editors will consider papers for publication based on novelty, their immediate or future impact on biotechnological processes, and their contribution to the advancement of biochemical engineering science. Submission of papers dealing with routine aspects of bioprocessing, description of established equipment, and routine applications of established methodologies (e.g., control strategies, modeling, experimental methods) is discouraged. Theoretical papers will be judged based on the novelty of the approach and their potential impact, or on their novel capability to predict and elucidate experimental observations.
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