Elucidation of Acetyl-Branched Sugar Pathway in Type II Polyketide Biosynthesis via One-Pot Enzymatic Synthesis

IF 5 1区化学 Q1 CHEMISTRY, ORGANIC

Organic Letters Pub Date : 2025-07-11 DOI:10.1021/acs.orglett.5c02185

Jia-Liang Zhou, Qiu-Yue Nie, Qiang Zhou and Gong-Li Tang*,

引用次数: 0

Abstract

Acetyl-branched sugars, although relatively rare, are found in certain natural products with notable bioactivities. Herein, we biochemically investigated the biosynthetic pathway of this rare sugar in type II polyketides featuring one-pot enzymatic synthesis of the key acetyl-branched TDP-sugar. The evidence shows that TjhB9/B10 were responsible for the attachment of an acetyl unit, a glycosyltransferase TjhB3 guided the uploading of a branched sugar, and a C7 reductase encoded by a gene outside the biosynthetic gene cluster subsequently reduced the TjhB3 product. Collectively, these results elucidated the sugar tailoring pathway of acetyl-branched sugar-modified polyketides.

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一锅法合成II型聚酮生物合成中乙酰支糖途径的研究。

乙酰支链糖虽然相对罕见，但在某些天然产物中发现具有显著的生物活性。本文以生物化学方法研究了II型多酮中这种稀有糖的生物合成途径，即一锅酶法合成关键的乙酰支链tdp -糖。有证据表明，TjhB9/B10负责乙酰基单元的附着，糖基转移酶TjhB3引导支链糖的上传，由生物合成基因簇外基因编码的C7还原酶随后还原TjhB3产物。总之，这些结果阐明了乙酰支链糖修饰聚酮的糖剪裁途径。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Organic Letters 化学-有机化学

CiteScore

9.30

自引率

11.50%

发文量

1607

审稿时长

1.5 months

期刊介绍： Organic Letters invites original reports of fundamental research in all branches of the theory and practice of organic, physical organic, organometallic,medicinal, and bioorganic chemistry. Organic Letters provides rapid disclosure of the key elements of significant studies that are of interest to a large portion of the organic community. In selecting manuscripts for publication, the Editors place emphasis on the originality, quality and wide interest of the work. Authors should provide enough background information to place the new disclosure in context and to justify the rapid publication format. Back-to-back Letters will be considered. Full details should be reserved for an Article, which should appear in due course.