STING expression in colorectal cancer: prognostic role and immune microenvironment link via mIHC.

IF 2.2 4区 医学 Q3 ONCOLOGY
Miao Zhang, Hualing Song, Xinju Zhou, Chunxian Zhou, Yajun Xu, Suyun Li
{"title":"STING expression in colorectal cancer: prognostic role and immune microenvironment link via mIHC.","authors":"Miao Zhang, Hualing Song, Xinju Zhou, Chunxian Zhou, Yajun Xu, Suyun Li","doi":"10.4149/neo_2025_250307N108","DOIUrl":null,"url":null,"abstract":"<p><p>Colorectal cancer (CRC) is a malignant tumor originating in the mucosal epithelium of the colon or rectum and is among the most common cancers of the digestive system. Now it is the fourth deadliest cancer in the world. Stimulator of Interferon Genes (STING) is a dimeric transmembrane protein on the endoplasmic reticulum membrane that induces the secretion of type I interferons and pro-inflammatory cytokines and is triggered by the cell membrane DNA of pathogens and hosts. It is widely expressed in immune cells (such as dendritic cells, macrophages) and some tumor cells, and has been shown to play a crucial role in the development of several tumors. However, issues such as the expression of STING in CRC and patient prognosis remain to be further elucidated. We investigated the expression of STING (in CRC tumor cells) and immune markers (CD3, CD4, CD8, CD56, CD163) in 86 CRC patients by tissue microarray using multicolor immunohistochemistry. Subsequently, the effect of STING on the tumor immune microenvironment was further explored. Finally, we analyzed the clinical significance of STING in CRC patients. We found elevated STING expression in tumor cells from CRC patients, which correlated with overall patient survival. High STING levels were associated with suppression of lymph node metastasis and reduction of CD163 tumor-associated macrophages. In contrast, upregulation of STING promoted infiltration of CD3+ T lymphocytes and CD8 lymphocyte subtype in the tumor microenvironment. Our study demonstrated that changes in STING expression in cancer cells in the tumor immune microenvironment have clinical significance and regulatory roles. STING-related functional mechanisms can be considered as therapeutic targets for CRC, which provides a theoretical basis for the subsequent clinical application of STING agonists.</p>","PeriodicalId":19266,"journal":{"name":"Neoplasma","volume":"72 3","pages":"184-191"},"PeriodicalIF":2.2000,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Neoplasma","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.4149/neo_2025_250307N108","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ONCOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Colorectal cancer (CRC) is a malignant tumor originating in the mucosal epithelium of the colon or rectum and is among the most common cancers of the digestive system. Now it is the fourth deadliest cancer in the world. Stimulator of Interferon Genes (STING) is a dimeric transmembrane protein on the endoplasmic reticulum membrane that induces the secretion of type I interferons and pro-inflammatory cytokines and is triggered by the cell membrane DNA of pathogens and hosts. It is widely expressed in immune cells (such as dendritic cells, macrophages) and some tumor cells, and has been shown to play a crucial role in the development of several tumors. However, issues such as the expression of STING in CRC and patient prognosis remain to be further elucidated. We investigated the expression of STING (in CRC tumor cells) and immune markers (CD3, CD4, CD8, CD56, CD163) in 86 CRC patients by tissue microarray using multicolor immunohistochemistry. Subsequently, the effect of STING on the tumor immune microenvironment was further explored. Finally, we analyzed the clinical significance of STING in CRC patients. We found elevated STING expression in tumor cells from CRC patients, which correlated with overall patient survival. High STING levels were associated with suppression of lymph node metastasis and reduction of CD163 tumor-associated macrophages. In contrast, upregulation of STING promoted infiltration of CD3+ T lymphocytes and CD8 lymphocyte subtype in the tumor microenvironment. Our study demonstrated that changes in STING expression in cancer cells in the tumor immune microenvironment have clinical significance and regulatory roles. STING-related functional mechanisms can be considered as therapeutic targets for CRC, which provides a theoretical basis for the subsequent clinical application of STING agonists.

STING在结直肠癌中的表达:通过mIHC的预后作用和免疫微环境联系。
结直肠癌(CRC)是一种起源于结肠或直肠粘膜上皮的恶性肿瘤,是消化系统最常见的癌症之一。现在它是世界上第四致命的癌症。干扰素基因刺激因子(STING)是一种位于内质网膜上的二聚体跨膜蛋白,可诱导I型干扰素和促炎细胞因子的分泌,由病原体和宿主细胞膜DNA触发。它在免疫细胞(如树突状细胞、巨噬细胞)和一些肿瘤细胞中广泛表达,并已被证明在几种肿瘤的发展中起着至关重要的作用。然而,STING在结直肠癌中的表达及患者预后等问题仍有待进一步阐明。采用多色免疫组织化学技术,应用组织芯片技术研究了86例结直肠癌患者肿瘤细胞中STING和免疫标志物(CD3、CD4、CD8、CD56、CD163)的表达情况。随后,我们进一步探讨了STING对肿瘤免疫微环境的影响。最后,我们分析STING在结直肠癌患者中的临床意义。我们在结直肠癌患者的肿瘤细胞中发现STING表达升高,这与患者的总体生存率相关。高水平的STING与抑制淋巴结转移和减少CD163肿瘤相关巨噬细胞有关。相反,STING上调可促进肿瘤微环境中CD3+ T淋巴细胞和CD8淋巴细胞亚型的浸润。我们的研究表明,肿瘤免疫微环境中癌细胞中STING表达的变化具有临床意义和调控作用。STING相关功能机制可作为结直肠癌的治疗靶点,为后续STING激动剂的临床应用提供理论依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Neoplasma
Neoplasma 医学-肿瘤学
CiteScore
5.40
自引率
0.00%
发文量
238
审稿时长
3 months
期刊介绍: The journal Neoplasma publishes articles on experimental and clinical oncology and cancer epidemiology.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信