Development and characterization of expressed sequence tag-simple sequence repeat markers for the near-threatened halophyte Artemisia fukudo Makino.

IF 1 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Yoshikuni Hodoki, Kako Ohbayashi, Naoko Ishikawa, Yuka Inoki, Nonoka Takehara
{"title":"Development and characterization of expressed sequence tag-simple sequence repeat markers for the near-threatened halophyte Artemisia fukudo Makino.","authors":"Yoshikuni Hodoki, Kako Ohbayashi, Naoko Ishikawa, Yuka Inoki, Nonoka Takehara","doi":"10.1266/ggs.25-00033","DOIUrl":null,"url":null,"abstract":"<p><p>RNA sequencing analysis was performed to develop 16 novel expressed sequence tag-simple sequence repeat (EST-SSR) markers to evaluate genetic variation in the near-threatened halophyte Artemisia fukudo Makino, which inhabits riversides and tidal muds affected by brackish water at high tide. In the four populations examined, the total number of alleles at each locus ranged from two to 13, with an average of 4.3. The observed and expected heterozygosity ranged from 0.05 to 0.64 and 0.06 to 0.72, respectively. These newly developed EST-SSR markers will support the understanding of the population genetic structure of A. fukudo and contribute to the conservation of this species.</p>","PeriodicalId":12690,"journal":{"name":"Genes & genetic systems","volume":" ","pages":""},"PeriodicalIF":1.0000,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Genes & genetic systems","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1266/ggs.25-00033","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

RNA sequencing analysis was performed to develop 16 novel expressed sequence tag-simple sequence repeat (EST-SSR) markers to evaluate genetic variation in the near-threatened halophyte Artemisia fukudo Makino, which inhabits riversides and tidal muds affected by brackish water at high tide. In the four populations examined, the total number of alleles at each locus ranged from two to 13, with an average of 4.3. The observed and expected heterozygosity ranged from 0.05 to 0.64 and 0.06 to 0.72, respectively. These newly developed EST-SSR markers will support the understanding of the population genetic structure of A. fukudo and contribute to the conservation of this species.

近危盐生植物福藤牧野蒿表达序列标记-简单序列重复标记的开发与鉴定。
利用RNA测序方法,建立了16个新的EST-SSR(表达序列标签-简单序列重复)标记,用于评价近危盐生植物福田蒿(Artemisia fukudo Makino)的遗传变异。福田蒿生长在涨潮时受咸淡水影响的河岸和潮汐泥中。在所检测的4个群体中,每个位点的等位基因总数为2 ~ 13个,平均为4.3个。观察到的杂合度为0.05 ~ 0.64,期望杂合度为0.06 ~ 0.72。这些新开发的EST-SSR标记将支持对福藤赤杨群体遗传结构的认识,为该物种的保护做出贡献。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Genes & genetic systems
Genes & genetic systems 生物-生化与分子生物学
CiteScore
1.50
自引率
0.00%
发文量
22
审稿时长
>12 weeks
期刊介绍: Genes & Genetic Systems , formerly the Japanese Journal of Genetics , is published bimonthly by the Genetics Society of Japan.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信