Lycium barbarum Polysaccharide Ameliorates Hindlimb Unloading-Induced Bone Loss by Targeting Noggin

IF 4.4 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

The FASEB Journal Pub Date : 2025-07-10 DOI:10.1096/fj.202403082R

Chuanxin Sun, Yuke He, Xia Chen, Kan Ding, Chang Chen

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Abstract

Disuse bone loss is a common metabolic bone disease with limited effective, safe treatments. Lycium barbarum (L. barbarum), a traditional Chinese medicine and popular health food, has been historically noted in Ben Cao Gang Mu (Compendium of Materia Medica) for its bone-strengthening properties. However, the effects of L. barbarum on disuse bone loss and the underlying mechanisms remain unclear. This study used a hindlimb-unloading mouse model and a simulated microgravity model of MC3T3-E1 cells in a rotary cell culture system to investigate the role of L. barbarum water extract (LBE) and polysaccharide (LBP). LBE and LBP significantly enhanced bone mass and strength in hindlimb-unloaded mice. Further analysis identified a polysaccharide component, LBPP, as the active ingredient mediating these effects. LBPP enhanced osteoblast differentiation and ossification under normal conditions and promoted osteoblast activity under simulated microgravity. Mechanistic studies revealed that LBPP directly binds to noggin, a potent inhibitor of BMPs, facilitating phosphorylation of downstream Smads to stimulate bone formation. These findings demonstrate the potential of L. barbarum as a functional food for preventing disuse bone loss and provide a theoretical basis for its therapeutic applications.

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枸杞多糖通过靶向Noggin改善后肢卸荷所致的骨丢失

废用性骨质流失是一种常见的代谢性骨病，有效、安全的治疗方法有限。枸杞（L. barbarum）是一种传统的中药和流行的保健食品，历史上被记载在《本草纲目》（本草纲目）中。然而，枸杞对废用性骨质流失的影响及其潜在机制尚不清楚。本研究采用小鼠后肢卸载模型和MC3T3-E1细胞在旋转细胞培养系统中的模拟微重力模型，探讨了枸杞水提取物（LBE）和多糖（LBP）的作用。LBE和LBP可显著提高后肢卸荷小鼠的骨量和强度。进一步分析发现多糖成分LBPP是介导这些作用的有效成分。LBPP在正常条件下促进成骨细胞分化和骨化，在模拟微重力条件下促进成骨细胞活性。机制研究表明，LBPP直接与noggin（一种有效的bmp抑制剂）结合，促进下游Smads的磷酸化以刺激骨形成。这些发现证明了枸杞作为一种预防废用性骨质流失的功能性食品的潜力，并为其治疗应用提供了理论基础。

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来源期刊

The FASEB Journal 生物-生化与分子生物学

CiteScore

9.20

自引率

2.10%

发文量

6243

审稿时长

3 months

期刊介绍： The FASEB Journal publishes international, transdisciplinary research covering all fields of biology at every level of organization: atomic, molecular, cell, tissue, organ, organismic and population. While the journal strives to include research that cuts across the biological sciences, it also considers submissions that lie within one field, but may have implications for other fields as well. The journal seeks to publish basic and translational research, but also welcomes reports of pre-clinical and early clinical research. In addition to research, review, and hypothesis submissions, The FASEB Journal also seeks perspectives, commentaries, book reviews, and similar content related to the life sciences in its Up Front section.